Rabbit anti-Human TK1 Polyclonal Antibody | anti-TK1 antibody

TK1 Antibody (Center)

Cat. Num. AAA9231016

• Gene Names: TK1; TK2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: TK1; Polyclonal Antibody; TK1 Antibody (Center); Thymidine kinase; cytosolic; 2.7.1.21; anti-TK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This TK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 139-173 amino acids from the Central region of human TK1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 234

• Applicable Applications for anti-TK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Cytoplasm.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-TK1 Antibody (Center) at 1:2000 dilutionLane 1: U-2OS whole cell lysateLane 2: SW620 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-TK1 antibody

Crown Antibodies; New Antibodies

References

Bradshaw H.D. Jr.,et al.Mol. Cell. Biol. 4:2316-2320 (1984). Flemington E.,et al.Gene 52:267-277 (1987). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Kreidberg J.A.,et al.Mol. Cell. Biol. 6:2903-2909 (1986).

NCBI and Uniprot Product Information

• NCBI GI #: 164698438
• NCBI GeneID: 7083
• NCBI Accession #: NP_003249.3
• NCBI GenBank Nucleotide #: NM_003258.4
• UniProt Accession #: P04183; Q969V0; Q9UMG9; B2RC58
• Molecular Weight: 25469 Da
• NCBI Official Full Name: thymidine kinase, cytosolic isoform 1
• NCBI Official Synonym Full Names: thymidine kinase 1
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic
• UniProt Protein Name: Thymidine kinase, cytosolic
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK1

NCBI Description

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

Uniprot Description

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: EC 2.7.1.21; Kinase, other; Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytosol

Molecular Function: identical protein binding; nucleoside kinase activity; protein binding; zinc ion binding

Biological Process: DNA metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; protein homotetramerization; pyrimidine nucleoside salvage; thymidine metabolic process