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Western Blot (WB) (Western blot analysis of extracts from COLO205 cells, using TK (Ab-13) antibody.)

Rabbit anti-Human TK Polyclonal Antibody | anti-TK antibody

TK (Ab-13) Antibody

Gene Names
TK1; TK2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
TK; Polyclonal Antibody; TK (Ab-13) Antibody; EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase; anti-TK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total TK protein
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
234
Applicable Applications for anti-TK antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-S(p)-P-S).
Target Name
TK
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from COLO205 cells, using TK (Ab-13) antibody.)

Western Blot (WB) (Western blot analysis of extracts from COLO205 cells, using TK (Ab-13) antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TK (Ab-13) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TK (Ab-13) antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells, using TK (Ab-13) antibody.)

Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 cells, using TK (Ab-13) antibody.)
Related Product Information for anti-TK antibody
Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell.TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division. Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive. These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response.N Singh, Mol. Cell. Biol., May 1991; 11: 2362 - 2374. Bin Tian, Am. J. Pathol., Aug 2003; 163: 789. Ren¨¦ Gerolami, Cancer Res., Feb 2000; 60: 993 - 1001. Catia Traversari, Blood, Jun 2007; 109: 4708 - 4715
Product Categories/Family for anti-TK antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,469 Da
NCBI Official Full Name
thymidine kinase, cytosolic
NCBI Official Synonym Full Names
thymidine kinase 1, soluble
NCBI Official Symbol
TK1
NCBI Official Synonym Symbols
TK2
NCBI Protein Information
thymidine kinase, cytosolic
UniProt Protein Name
Thymidine kinase, cytosolic
Protein Family
UniProt Gene Name
TK1
UniProt Entry Name
KITH_HUMAN

Uniprot Description

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other; EC 2.7.1.21

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; fetal process involved in parturition; protein homotetramerization; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; response to copper ion; gut development; pyrimidine base metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage; thymidine metabolic process; phosphorylation

Research Articles on TK

Similar Products

Product Notes

The TK tk1 (Catalog #AAA9401946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TK (Ab-13) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the TK tk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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