Immunohistochemistry (IHC)
(IHC image of MBS7134223 diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human TJP2 Polyclonal Antibody | anti-TJP2 antibody
TJP2 Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IHC: 1:500-1:1000
IF: 1:200-1:500
Immunohistochemistry (IHC)
(IHC image of MBS7134223 diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7134223 diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with MBS7134223 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with MBS7134223 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, K562 whole cell lysateAll lanes: TJP2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 134, 118, 112, 132, 116, 131, 138 kDaObserved band size: 150 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, K562 whole cell lysateAll lanes: TJP2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 134, 118, 112, 132, 116, 131, 138 kDaObserved band size: 150 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
ZO2: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with epithelial tight junctions (TJ). May mediate dynamic changes in the composition of TJs. Plays a role in tight junctions and adherens junctions. Forms homodimers, and heterodimer with ZO-1 and ZO-3. The ZOs shuttle between the TJ and the nucleus, where they may regulate gene expression. Interacts with occluding, UBN1 and scribble. Interacts with SAFB in the nucleus. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Five isoforms of the human protein are produced by alternative promoters. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues..
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 9q13-q21
Cellular Component: nucleoplasm; tight junction; adherens junction; cytoplasm; plasma membrane; cell junction; cytosol
Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; guanylate kinase activity; protein binding
Biological Process: response to organic substance; apoptosis; cell structure disassembly during apoptosis; nucleotide phosphorylation; intestinal absorption
Disease: Hypercholanemia, Familial; Cholestasis, Progressive Familial Intrahepatic, 4
Research Articles on TJP2
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Product Notes
The TJP2 tjp2 (Catalog #AAA7134223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TJP2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TJP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:500-1:1000 IF: 1:200-1:500. Researchers should empirically determine the suitability of the TJP2 tjp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TJP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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