Western Blot (WB)
(HEK293 overexpressing TFPI (RC219033) and probed (mock transfection in first lane), tested by Origene.)
Goat Tissue Factor Pathway Inhibitor Polyclonal Antibody | anti-TFPI antibody
Goat anti-Tissue Factor Pathway Inhibitor Antibody
Western Blot: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35. 0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx.38kDa to 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
(HEK293 overexpressing TFPI (RC219033) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing TFPI (RC219033) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(Staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.3ug/ml) of Hela lysate (RIPA buffer, 35ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
Uniprot Description
TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 2q32
Cellular Component: cell surface; endoplasmic reticulum; extracellular region; extracellular space; plasma membrane
Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity
Biological Process: blood coagulation; blood coagulation, extrinsic pathway
Research Articles on TFPI
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Product Notes
The TFPI tfpi (Catalog #AAA420689) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Tissue Factor Pathway Inhibitor Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tissue Factor Pathway Inhibitor can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 50kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35. 0kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx.38kDa to 45kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TFPI tfpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VKIAYEEIFV KNM. It is sometimes possible for the material contained within the vial of "Tissue Factor Pathway Inhibitor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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