Western Blot (WB)
(Western Blot: Sample: Human 304 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine NOS3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Cattle Nitric Oxide Synthase 3 Polyclonal Antibody | anti-NOS3 antibody
Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATILYASET GRAQSYAQQL GRLFRKAFDP RVLCMDEYDV VSLEHEALVL VVTSTFGNGD PPENGESFAA ALMEMSGPYN SSPRPEQHKS YKIRFNSVSC SDPLVSSWRR KRKESSNTDS AGALGTLRFC VFGLGSRAYP
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human 304 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine NOS3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human 304 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine NOS3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
Uniprot Description
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase
Cellular Component: caveola; cytoskeleton; Golgi apparatus
Molecular Function: calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity
Biological Process: arginine catabolic process; blood coagulation; cellular response to laminar fluid shear stress; mitochondrion organization; negative regulation of blood pressure; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of guanylate cyclase activity
Research Articles on NOS3
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Product Notes
The NOS3 nos3 (Catalog #AAA2028121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATILYASET GRAQSYAQQL GRLFRKAFDP RVLCMDEYDV VSLEHEALVL VVTSTFGNGD PPENGESFAA ALMEMSGPYN SSPRPEQHKS YKIRFNSVSC SDPLVSSWRR KRKESSNTDS AGALGTLRFC VFGLGSRAYP. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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