Goat anti-Mouse TIP47 Polyclonal Antibody

TIP47, ID (M6PRBP1, Perilipin-3, 47kD Mannose 6-phosphate Receptor-binding Protein, 47kD MPR-binding Protein, Cargo Selection Protein TIP47, Mannose-6-phosphate Receptor-binding Protein 1, Placental Protein 17, PP17, PLIN3, M6PRBP1)

Cat. Num. AAA6005543

• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TIP47; Polyclonal Antibody; ID (M6PRBP1; Perilipin-3; 47kD Mannose 6-phosphate Receptor-binding Protein; 47kD MPR-binding Protein; Cargo Selection Protein TIP47; Mannose-6-phosphate Receptor-binding Protein 1; Placental Protein 17; PP17; PLIN3; M6PRBP1); Anti -TIP47; anti-TIP47 antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Recognizes mouse M6PRBP1. Species sequence homology: Rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-TIP47 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Western Blot: 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-NQKTPQDAEKDPAK of mouse M6PRBP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Mouse Spleen (formalin-fixed, paraffin-embedded) stained with M6PRBP1 antibody followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Mouse Testis (formalin-fixed, paraffin-embedded) stained with M6PRBP1 antibody followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Testing Data

(Antibody (0.1ug/ml) staining of Rat Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TIP47 antibody

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin. It has been described as a placental protein, as well. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, this protein is probably significant as an oncodevelopmental marker.

Product Categories/Family for anti-TIP47 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 346969694
• NCBI Official Full Name: TIP47