Rabbit TIMP-1 Polyclonal Antibody | anti-TIMP-1 antibody

RABBIT ANTI HUMAN TIMP-1

Cat. Num. AAA224268

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: TIMP-1; Polyclonal Antibody; RABBIT ANTI HUMAN TIMP-1; anti-TIMP-1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-TIMP-1 antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: This purified human TIMP-1 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human TIMP-1 antibody as the detection reagent and recombinant human TIMP-1 as the standard.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Immunohistology - Paraffin: Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose. ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antiserum to human TIMP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human TIMP-1 (MBS224268) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #2

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human TIMP-1 (MBS224268) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #3

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human TIMP-1 (MBS224268) following enzyme mediated antigen retrieval using proteinase K)

Related Product Information for anti-TIMP-1 antibody

Rabbit anti Human TIMP-1 antibody specifically recognizes human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 4507509
• NCBI GeneID: 7076
• NCBI Accession #: NP_003245.1
• NCBI GenBank Nucleotide #: NM_003254.2
• UniProt Accession #: P01033; Q14252; Q9UCU1
• Molecular Weight: 23,171 Da
• NCBI Official Full Name: metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP
• NCBI Protein Information: metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1
• UniProt Entry Name: TIMP1_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity

Biological Process: response to peptide hormone stimulus; extracellular matrix disassembly; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; blood coagulation; aging; negative regulation of apoptosis