Rabbit anti-Human TIM3 Polyclonal Antibody | anti-HAVCR2 antibody

TIM3 Antibody

Cat. Num. AAA154462

• Gene Names: HAVCR2; TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: TIM3; Polyclonal Antibody; TIM3 Antibody; Hepatitis A virus cellular receptor; HAVCR2; TIM-3; CD366; KIM-3; TIMD3; TIMD-3; hepatitis A virus cellular receptor 2; anti-HAVCR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two antibodies are known to exist; this antibody will detect both isoforms.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 142

• Applicable Applications for anti-HAVCR2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TIM3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM3.; The immunogen is located within amino acids 60 - 110 of TIM3.
• Buffer: TIM3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TIM-3 in human spleen tissue with TIM-3 antibody at 2 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TIM-3 in human spleen tissue with TIM-3 antibody at 20 μg/ml.Red: TIM-3 Antibody (8659)Blue: DAPI staining)

Related Product Information for anti-HAVCR2 antibody

The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).

Product Categories/Family for anti-HAVCR2 antibody

Cell Cycle; Cancer; Immunology; Immune Checkpoint

References

1. Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41. 2. Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187-94. 3. Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501-10.

NCBI and Uniprot Product Information

• NCBI GI #: 4957453
• NCBI GeneID: 84868
• UniProt Accession #: Q8TDQ0; Q8WW60; Q96K94; B2RAY2
• Molecular Weight: Predicted: 33 kDa; Observed: 37 kDa
• NCBI Official Full Name: Drosophila melanogaster genome survey sequence T7 end of BAC: BACR18K08 of RPCI-98 library from Drosophila melanogaster (fruit fly), genomic survey sequence
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 2
• NCBI Official Symbol: HAVCR2
• NCBI Official Synonym Symbols: TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• NCBI Protein Information: hepatitis A virus cellular receptor 2
• UniProt Protein Name: Hepatitis A virus cellular receptor 2
• Protein Family: Hepatitis A virus cellular receptor
• UniProt Gene Name: HAVCR2
• UniProt Synonym Gene Names: TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Uniprot Description

Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock ().