Rabbit anti-Human TIGAR Polyclonal Antibody | anti-TIGAR antibody

TIGAR Antibody

Cat. Num. AAA9612080

• Gene Names: TIGAR; FR2BP; C12orf5
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TIGAR; Polyclonal Antibody; TIGAR Antibody; 6-bisphosphatase TIGAR; C12ORF5; chromosome 12 open reading frame 5; FR2BP; Fructose-2; 6-bisphosphate 2-phosphatase; Probable fructose 2; 6 bisphosphatase TIGAR; Probable fructose-2; tigar; TIGAR_HUMAN; TP53 induced glycolysis and apoptosis regulator; TP53 induced glycolysis regulatory phosphatase; TP53-induced glycolysis and apoptosis regulator; Transactivated by NS3TP2 protein; anti-TIGAR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIGAR Antibody detects endogenous levels of total TIGAR.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 270

• Applicable Applications for anti-TIGAR antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TIGAR, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using TIGAR Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612080 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612080 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-TIGAR antibody

Function: Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (By similarity). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (By similarity). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973).

Subcellular Location: Cytoplasm. Nucleus. Mitochondrion. Note: Translocated to the mitochondria during hypoxia in a HIF1A-dependent manner (PubMed:23185017). Colocalizes with HK2 in the mitochondria during hypoxia (PubMed:23185017). Translocated to the nucleus during hypoxia and/or genome stress-induced DNA damage responses in cancer cells (PubMed:25928429). Translocation to the mitochondria is enhanced in ischemic cortex after reperfusion and/or during oxygen and glucose deprivation (OGD)/reoxygenation insult in primary neurons (By similarity).

Tissue Specificity: Expressed in the brain (PubMed:22887998). Expressed in breast tumors (PubMed:21820150). Expressed in glioblastomas (PubMed:22887998).

Subunit Structure: Interacts with HK2; the interaction increases hexokinase HK2 activity in a hypoxia- and HIF1A-dependent manner, resulting in the regulation of mitochondrial membrane potential, thus increasing NADPH production and decreasing intracellular ROS levels (PubMed:23185017).

Similarity: Belongs to the phosphoglycerate mutase family.

NCBI and Uniprot Product Information

• NCBI GI #: 9966849
• NCBI GeneID: 57103
• NCBI Accession #: NP_065108.1
• NCBI GenBank Nucleotide #: NP_065108.1
• UniProt Accession #: Q9NQ88
• Molecular Weight: Observed Molecular Weight: (Observed)30kD.; Predicted Molecular Weight: (Calculated)30kDa.
• NCBI Official Full Name: fructose-2,6-bisphosphatase TIGAR
• NCBI Official Synonym Full Names: TP53 induced glycolysis regulatory phosphatase
• NCBI Official Symbol: TIGAR
• NCBI Official Synonym Symbols: FR2BP; C12orf5
• NCBI Protein Information: fructose-2,6-bisphosphatase TIGAR
• UniProt Protein Name: Fructose-2,6-bisphosphatase TIGAR
• Protein Family: Fructose-2,6-bisphosphatase
• UniProt Gene Name: TIGAR
• UniProt Synonym Gene Names: C12orf5
• UniProt Entry Name: TIGAR_HUMAN

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIGAR: Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. Belongs to the phosphoglycerate mutase family.

Protein type: EC 3.1.3.46; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: intracellular

Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity

Biological Process: response to xenobiotic stimulus; dephosphorylation; response to gamma radiation