Fructose-2,6-bisphosphatase TIGAR protein (TIGAR) Active Protein | TIGAR active protein

Recombinant Human Fructose-2,6-bisphosphatase TIGAR protein (TIGAR) (Active)

Cat. Num. AAA969129

• Gene Names: TIGAR; FR2BP; C12orf5
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Fructose-2; 6-bisphosphatase TIGAR protein (TIGAR); Recombinant Human Fructose-2; 6-bisphosphatase TIGAR protein (TIGAR) (Active); EC 3.1.3.46; TP53-induced glycolysis regulatory phosphatase; TIGAR active protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized
• Sequence Positions: 1-270aa
• Sequence: MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR+GGYGRKKRRQ
• Sequence Length: 270

• Species: Homo sapiens (Human)
• Biological Activity: Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 ug/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Protein Note: Protein will be provided with aseptic processing and endotoxin removal.
• Preparation and Storage: Generally, the shelf life of liquid form is 6 months at -20/-80 degree C. The shelf life of lyophilized form is 12 months at -20/-80 degree C.

SDS-PAGE

Related Product Information for TIGAR active protein

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (By similarity). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (By similarity). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973). {ECO:0000250|UniProtKB:Q8BZA9, ECO:0000269|PubMed:16839880, ECO:0000269|PubMed:19015259, ECO:0000269|PubMed:19713938, ECO:0000269|PubMed:22887998, ECO:0000269|PubMed:23185017, ECO:0000269|PubMed:23726973, ECO:0000269|PubMed:23817040, ECO:0000269|PubMed:25928429}.

Product Categories/Family for TIGAR active protein

Biological Active Protein; Cell Biology; Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 9966849
• NCBI GeneID: 57103
• NCBI Accession #: NP_065108.1
• NCBI GenBank Nucleotide #: NM_020375.2
• UniProt Accession #: Q9NQ88; B2R840
• Molecular Weight: 31.7 kDa
• NCBI Official Full Name: fructose-2,6-bisphosphatase TIGAR
• NCBI Official Synonym Full Names: TP53 induced glycolysis regulatory phosphatase
• NCBI Official Symbol: TIGAR
• NCBI Official Synonym Symbols: FR2BP; C12orf5
• NCBI Protein Information: fructose-2,6-bisphosphatase TIGAR
• UniProt Protein Name: Fructose-2,6-bisphosphatase TIGAR
• Protein Family: Fructose-2,6-bisphosphatase
• UniProt Gene Name: TIGAR

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIGAR: Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. Belongs to the phosphoglycerate mutase family.

Protein type: EC 3.1.3.46; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: cytoplasm; cytosol; intracellular; mitochondrial outer membrane; nucleus

Molecular Function: bisphosphoglycerate phosphatase activity; fructose-2,6-bisphosphate 2-phosphatase activity; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 2,6-bisphosphate metabolic process; negative regulation of programmed cell death; positive regulation of DNA repair; regulation of pentose-phosphate shunt; response to DNA damage stimulus