Western Blot (WB)
(Western Blot analysis of COLO205 cells using TIF1a Polyclonal Antibody)
Rabbit anti-Human, Mouse TIF1alpha Polyclonal Antibody | anti-TIF1alpha antibody
TIF1alpha Polyclonal Antibody
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:20000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of COLO205 cells using TIF1a Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using TIF1a Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of NIH-3T3 cells using TIF1a Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of NIH-3T3 cells using TIF1a Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.
Protein type: Protein kinase, atypical; Tumor suppressor; Ubiquitin conjugating system; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; EC 6.3.2.-; Kinase, protein; ATYPICAL group; TIF1 family
Chromosomal Location of Human Ortholog: 7q32-q34
Cellular Component: nucleoplasm; perichromatin fibrils; cytosol; nucleus
Molecular Function: ligand-dependent nuclear receptor binding; protein binding; zinc ion binding; sequence-specific DNA binding; p53 binding; transcription coactivator activity; ubiquitin-protein ligase activity; estrogen response element binding; chromatin binding; methylated histone residue binding; receptor binding; protein kinase activity; ligase activity
Biological Process: calcium ion homeostasis; regulation of apoptosis; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; response to peptide hormone stimulus; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; regulation of protein stability; protein ubiquitination; protein catabolic process; negative regulation of transcription, DNA-dependent
Disease: Thyroid Carcinoma, Papillary
Research Articles on TIF1alpha
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Product Notes
The TIF1alpha trim24 (Catalog #AAA9414090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIF1alpha Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIF1alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the TIF1alpha trim24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIF1alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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