Rabbit anti-Human TIEG Polyclonal Antibody | anti-TIEG antibody

TIEG, ID (KLF10, TIEG, TIEG1, Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1) (HRP)

Cat. Num. AAA6355540

• Gene Names: KLF10; EGRA; TIEG; TIEG1; EGR-alpha
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: TIEG; Polyclonal Antibody; ID (KLF10; TIEG1; Krueppel-like factor 10; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1) (HRP); KLF10; Transforming growth factor-beta-inducible early growth response protein 1; anti-TIEG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TIEG antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-233 amino acids from the Central region of human TIEG.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of TIEG (arrow) using rabbit polyclonal TIEG Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TIEG gene.)

Flow Cytometry (FC/FACS)

(TIEG Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TIEG antibody

Antibodies; Growth Factors; Cytokines

References

Kim, J., et al. Biochem. Biophys. Res. Commun. 401(3):412-416(2010). Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010). Subramaniam, M., et al. Biofactors 36(1):8-18(2010). Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010): Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5032177
• NCBI GeneID: 7071
• NCBI Accession #: NP_005646
• NCBI GenBank Nucleotide #: NM_005655
• UniProt Accession #: Q13118
• Molecular Weight: 52kDa
• NCBI Official Full Name: Krueppel-like factor 10 isoform a
• NCBI Official Synonym Full Names: Kruppel like factor 10
• NCBI Official Symbol: KLF10
• NCBI Official Synonym Symbols: EGRA; TIEG; TIEG1; EGR-alpha
• NCBI Protein Information: Krueppel-like factor 10
• UniProt Protein Name: Krueppel-like factor 10
• UniProt Gene Name: KLF10
• UniProt Synonym Gene Names: TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]