Western Blot (WB)
(Western blot shows lysates of NIH3T3 transfected with mouse Tie2, untreated (-) or treated (+) with 600ng/ml Recombinant Human Angiopoietin1 for 5 minutes. PVDF membrane was probed with 1ug/ml MBS612813 followed by HRP-conjugated Anti-Rabbit IgG. A specific band for Tie2 was detected at ~160kD (as indicated). This experiment was conducted under reducing conditions.)
Rabbit anti-Human, Mouse Tie2 Polyclonal Antibody | anti-TEK antibody
Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml detected Tie2 in immersion fixed paraffin embedded sections of mouse embryonic brain and human placenta.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot shows lysates of NIH3T3 transfected with mouse Tie2, untreated (-) or treated (+) with 600ng/ml Recombinant Human Angiopoietin1 for 5 minutes. PVDF membrane was probed with 1ug/ml MBS612813 followed by HRP-conjugated Anti-Rabbit IgG. A specific band for Tie2 was detected at ~160kD (as indicated). This experiment was conducted under reducing conditions.)
Western Blot (WB)
(Western blot shows lysates of NIH3T3 transfected with mouse Tie2, untreated (-) or treated (+) with 600ng/ml Recombinant Human Angiopoietin1 for 5 minutes. PVDF membrane was probed with 1ug/ml MBS612813 followed by HRP-conjugated Anti-Rabbit IgG. A specific band for Tie2 was detected at ~160kD (as indicated). This experiment was conducted under reducing conditions.)
Immunohistochemistry (IHC)
(Tie2 in Human Placenta. Tie2 phosphorylated at Y1102 was detected in immersion fixed paraffin embedded sections of human placenta using MBS612813 at 15ug/ml overnight at 4ºC. Tissue was stained using the Anti-Rabbit HRP DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).)
Immunohistochemistry (IHC)
(Tie2 in Human Placenta. Tie2 phosphorylated at Y1102 was detected in immersion fixed paraffin embedded sections of human placenta using MBS612813 at 15ug/ml overnight at 4ºC. Tissue was stained using the Anti-Rabbit HRP DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).)
NCBI and Uniprot Product Information
NCBI Description
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]
Uniprot Description
TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; TK group; Tie family
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: microvillus; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; stress fiber
Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity
Biological Process: response to peptide hormone stimulus; response to cAMP; peptidyl-tyrosine phosphorylation; cell-matrix adhesion; heart development; protein amino acid autophosphorylation; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Disease: Venous Malformations, Multiple Cutaneous And Mucosal
Research Articles on TEK
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Product Notes
The TEK tek (Catalog #AAA612813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tie2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml detected Tie2 in immersion fixed paraffin embedded sections of mouse embryonic brain and human placenta. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Researchers should empirically determine the suitability of the TEK tek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tie2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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