Western Blot (WB)
(Anti-TIE2 Picoband antibody, -1.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: Recombinant Human TIE2 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)
anti-Human TIE2 Polyclonal Antibody | anti-TIE2 antibody
Anti-TIE2 Antibody
Western Blot (WB)
(Anti-TIE2 Picoband antibody, -1.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: Recombinant Human TIE2 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)
Western Blot (WB)
(Anti-TIE2 Picoband antibody, -1.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: Recombinant Human TIE2 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)
Western Blot (WB)
(Anti-TIE2 Picoband antibody, -2.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD)
Western Blot (WB)
(Anti-TIE2 Picoband antibody, -2.jpgAll lanes: Anti TIE2 at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 125KDObserved bind size: 125KD)
Background: TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
Uniprot Description
TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Protein type: EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Tie family
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: actin filament; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cell surface; cytoplasm; extracellular region; focal adhesion; integral to plasma membrane; intercellular junction; lipid raft; microvillus; plasma membrane; stress fiber
Molecular Function: ATP binding; growth factor binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: angiogenesis; cell-cell signaling; endochondral ossification; endothelial cell proliferation; heart development; leukocyte migration; MAPKKK cascade; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of inflammatory response; organ regeneration; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; protein oligomerization; regulation of establishment and/or maintenance of cell polarity; regulation of vascular permeability; response to cAMP; response to estrogen stimulus; response to hypoxia; response to peptide hormone stimulus; signal transduction; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Venous Malformations, Multiple Cutaneous And Mucosal
Research Articles on TIE2
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Product Notes
The TIE2 tek (Catalog #AAA177820) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIE2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIE2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the TIE2 tek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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