Human TIE2 ELISA Kit | TEK elisa kit

Human TIE2 PicoKine ELISA Kit

Cat. Num. AAA175906

• Gene Names: TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
• Reactivity: Human
• Synonyms: TIE2; Human TIE2 PicoKine ELISA Kit; Angiopoietin-1 receptor; TEK tyrosine kinase; endothelial; CD202b; CD202b antigen; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; Tek; TIE 2; TIE2_HUMAN; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE 2; Tyrosine-protein kinase receptor TIE-2; venous malformations; multiple cutaneous and mucosal; VMCM; VMCM1; TEK elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TEK
• Sequence Length: 1124

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <5pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TEK elisa kit

Principle of the Assay: The Picokine™ Human TEK Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TEK with a 96-well strip plate that is pre-coated with antibody specific for TEK. The detection antibody is a biotinylated antibody specific for TEK. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TEK with immunogen: Expression system for standard: NSO; Immunogen sequence: A23-K745. The kit is analytically validated with ready to use reagents. To measure Human TEK, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human TEK in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TEK in the sample.

Background/Introduction: Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 218511853
• NCBI GeneID: 7010
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 109,141 Da
• NCBI Official Full Name: Angiopoietin-1 receptor
• NCBI Official Synonym Full Names: TEK tyrosine kinase, endothelial
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2
• UniProt Protein Name: Angiopoietin-1 receptor
• Protein Family: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN

NCBI Description

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: focal adhesion; microvillus; cell surface; integral to plasma membrane; basolateral plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; basal plasma membrane; stress fiber; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; growth factor binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; response to cAMP; peptidyl-tyrosine phosphorylation; cell-matrix adhesion; protein amino acid autophosphorylation; heart development; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal