Rabbit Tie-2 Polyclonal Antibody | anti-Tie-2 antibody

Tie-2 (Phospho-Y1108) Polyclonal Antibody

Cat. Num. AAA3005450

• Gene Names: TEK; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Tie-2; Polyclonal Antibody; Tie-2 (Phospho-Y1108) Polyclonal Antibody; Angiopoietin-1 receptor; Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD202b; TEK; TIE2; VMCM; VMCM1; anti-Tie-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Tie-2 (Y1108) polyclonal antibody detects endogenous levels of Tie-2 protein when phosphorylated at Tyr1108.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1124

• Applicable Applications for anti-Tie-2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Tie-2 around the phosphorylation site of Tyrosine 1108.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Tie-2 (Y1108) polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysate treated with PMA (100nM,15mins) Lane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-Tie-2 antibody

Receptor tyrosine kinases play key roles in signal transduction across cell surfaces in biological systems, including the vascular system. These receptors comprise a large and diverse family of catalytically related proteins that, on the basis of sequence and structural similarities, can be divided into several different evolutionary subfamilies. The cloning and characterization of Tie-1 (also designated Tie), a novel human endothelial cell surface receptor tyrosine kinase, has been reported. The extracellular domain of the predicted Tie-1 protein product has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. An additional member of this family has been identified as Tie-2 (also designated Tek). Tie-1 and Tie-2 have been shown to be encoded by distinct genes and to represent members of a new class of receptor tyrosine kinases.

NCBI and Uniprot Product Information

• NCBI GI #: 88758596
• NCBI GeneID: 7010
• NCBI Accession #: NP_000450.2
• NCBI GenBank Nucleotide #: NP_000450.2
• UniProt Accession #: Q02763
• Molecular Weight: ~ 160kDa
• NCBI Official Full Name: angiopoietin-1 receptor isoform 1
• NCBI Official Synonym Full Names: TEK receptor tyrosine kinase
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor
• UniProt Protein Name: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN

NCBI Description

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Membrane protein, integral; EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: microvillus; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; stress fiber; basal plasma membrane

Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; cell-matrix adhesion; heart development; protein amino acid autophosphorylation; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal