Goat TIAM2 Polyclonal Antibody | anti-TIAM2 antibody

Goat anti-TIAM2 Antibody

Cat. Num. AAA422767

• Gene Names: TIAM2; STEF; TIAM-2
• Reactivity: Expected from sequence similarity: Human, Mouse, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TIAM2; Polyclonal Antibody; Goat anti-TIAM2 Antibody; Goat Anti-TIAM2 Antibody; T-cell lymphoma invasion and metastasis 2; FLJ41865; STEF; TIAM-2; OTTHUMP00000017472; SIF and TIAM1-like exchange factor; T-lymphoma invasion and metastasis-inducing protein 2; TIAM2 antibody; T-cell lymphoma invasion and metastasis 2 antibody; FLJ41865 antibody; STEF antibody; TIAM-2 antibody; OTTHUMP00000017472 antibody; SIF and TIAM1-like exchange factor antibody; T-lymphoma invasion and metastasis-inducing protein 2 antibody; anti-TIAM2 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QIRHQSLDSQSEN
• Sequence Length: 1701

• Applicable Applications for anti-TIAM2 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 70kDa band in NIH3T3 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to Mouse NP_036008.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-QIRHQSLDSQSEN, from the internal region of the protein sequence according to NP_036586.2; NP_001010927.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize all reported isoforms (NP_036586.2; NP_001010927.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 58331187
• NCBI GeneID: 26230
• NCBI Accession #: NP_036586.2; NP_001010927.1
• NCBI GenBank Nucleotide #: NM_012454.3
• Molecular Weight: 192,555 Da
• NCBI Official Full Name: T-lymphoma invasion and metastasis-inducing protein 2 isoform a
• NCBI Official Synonym Full Names: T-cell lymphoma invasion and metastasis 2
• NCBI Official Symbol: TIAM2
• NCBI Official Synonym Symbols: STEF; TIAM-2
• NCBI Protein Information: T-lymphoma invasion and metastasis-inducing protein 2
• UniProt Protein Name: T-lymphoma invasion and metastasis-inducing protein 2
• Protein Family: T-lymphoma invasion and metastasis-inducing protein
• UniProt Gene Name: TIAM2
• UniProt Synonym Gene Names: KIAA2016; STEF; TIAM-2
• UniProt Entry Name: TIAM2_HUMAN

NCBI Description

This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

TIAM2: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane. Activates specifically RAC1, but not CDC42 and RHOA. Belongs to the TIAM family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 6q25.2

Cellular Component: cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of apoptosis; regulation of small GTPase mediated signal transduction