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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Thy1 Cell Surface Antigen (Thy1) Polyclonal Antibody | anti-Thy1 antibody

Polyclonal Antibody to Thy1 Cell Surface Antigen (Thy1)

Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Thy1 Cell Surface Antigen (Thy1); Polyclonal Antibody; Polyclonal Antibody to Thy1 Cell Surface Antigen (Thy1); anti-Thy1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Thy1. It has been selected for its ability to recognize Thy1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.51mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- Q KVTSLTACLV NQNLRLDCRH ENNTKDNSIQ HEFSLTREKR KHVLSGTLGI PEHTYRSRVT LSNQPYIKVL TLANFTTKDE GDYFCELQVS GANPMSSNKS ISVYRDKLVK C
Sequence Length
161
Applicable Applications for anti-Thy1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant Thy1 (Gln20~Cys131) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054808
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:MouseTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:MouseTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,080 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
thymus cell antigen 1, theta
NCBI Official Symbol
Thy1
NCBI Official Synonym Symbols
T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
Thy1
UniProt Synonym Gene Names
Thy-1

NCBI Description

This gene encodes a glycoprotein that is anchored to the cell surface of thymocytes, neuronal and other cells through a glycosyl-phosphatidylinositol moiety. A soluble form of the encoded protein has also been detected in serum and cerebrospinal fluid. The encoded protein undergoes further processing to generate the mature protein which mediates cell-cell interactions to trigger downstream signaling pathways. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9 A5.1|9 24.52 cM

Cellular Component: anchored component of external side of plasma membrane; anchored to plasma membrane; apical plasma membrane; axolemma; cell surface; cytosol; dendrite; endoplasmic reticulum; external side of plasma membrane; focal adhesion; growth cone; membrane; membrane raft; myelin sheath; plasma membrane

Molecular Function: enzyme binding; GTPase activator activity; integrin binding; protein kinase binding

Biological Process: angiogenesis; cell adhesion; cell-cell signaling; cytoskeleton organization; focal adhesion assembly; integrin-mediated signaling pathway; negative regulation of axonogenesis; negative regulation of cell migration; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; positive regulation of cellular extravasation; positive regulation of focal adhesion assembly; positive regulation of GTPase activity; positive regulation of heterotypic cell-cell adhesion; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; protein autophosphorylation; receptor clustering; regulation of cell migration; regulation of cell-matrix adhesion; retinal cone cell development; T cell receptor signaling pathway

Research Articles on Thy1

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Product Notes

The Thy1 thy1 (Catalog #AAA2004276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Thy1 Cell Surface Antigen (Thy1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Thy1 Cell Surface Antigen (Thy1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Thy1 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- Q KVTSLTACLV NQNLRLDCRH ENNTKDNSIQ HEFSLTREKR KHVLSGTLGI PEHTYRSRVT LSNQPYIKVL TLANFTTKDE GDYFCELQVS GANPMSSNKS ISVYRDKLVK C. It is sometimes possible for the material contained within the vial of "Thy1 Cell Surface Antigen (Thy1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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