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SDS-Page

Gamma-Glutamyltransferase 5 Recombinant Protein | gGT5 recombinant protein

Recombinant Gamma-Glutamyl transferase 5 (gGT5)

Gene Names
Ggt5; Ggta1; Ggtla1
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Gamma-Glutamyltransferase 5; Recombinant Gamma-Glutamyl transferase 5 (gGT5); gGT5 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 95%
Form/Format
Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
Concentration
Original Concentration: 100ug/mL (varies by lot)
Sequence
TSH VSVLGEDGSA VAATSTINTP FGAMVYSPRT GIILNNELLD LCERCPRGSG TTPSPVSGDR VGGAPGRCWP PVPGERSPSS MVPSILINKA QGSKLVIGGA GGELIISAVA QAIMSKLWLG FDLRAAIAAP ILHVNSKGCV EYEPNFSQEV QRGLQDRGQN QTQRPFFLNVVQAVSQEGAC VYAVSDLRKS GEAAGY
Sequence Length
572
Applicable Applications for gGT5 recombinant protein
Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
Application Notes
May be suitable for use in other assays to be determined by the end user.
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Thr388~Tyr586
Tags
N-terminal His Tag
Subcellular Location
Membrane
Predicted isoelectric point
6.7
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 24.5kDa
Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
gamma-glutamyltransferase 5
NCBI Official Synonym Full Names
gamma-glutamyltransferase 5
NCBI Official Symbol
Ggt5
NCBI Official Synonym Symbols
Ggta1; Ggtla1
NCBI Protein Information
gamma-glutamyltransferase 5
UniProt Protein Name
Gamma-glutamyltransferase 5
Protein Family
UniProt Gene Name
Ggt5
UniProt Synonym Gene Names
Ggtla1; GGT 5; GGL; GGT-rel
UniProt Entry Name
GGT5_RAT

NCBI Description

involved in protecting the lungs from stress due to oxidation or other types of toxics [RGD, Feb 2006]

Uniprot Description

GGT5: Cleaves the gamma-glutamyl peptide bond of glutathione conjugates, but maybe not glutathione itself. Converts leukotriene C4 (LTC4) to leukotriene D4 (LTD4). Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; EC 2.3.2.2; EC 3.4.19.14; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; EC 3.4.19.13; Other Amino Acids Metabolism - glutathione

Cellular Component: plasma membrane; integral to membrane

Molecular Function: gamma-glutamyltransferase activity

Biological Process: leukotriene biosynthetic process; glutathione biosynthetic process; response to oxidative stress; proteolysis; inflammatory response; response to organic nitrogen

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Product Notes

The gGT5 ggt5 (Catalog #AAA2031426) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Gamma-Glutamyltransferase 5 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB). May be suitable for use in other assays to be determined by the end user. Researchers should empirically determine the suitability of the gGT5 ggt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TSH VSVLGEDGSA VAATSTINTP FGAMVYSPRT GIILNNELLD LCERCPRGSG TTPSPVSGDR VGGAPGRCWP PVPGERSPSS MVPSILINKA QGSKLVIGGA GGELIISAVA QAIMSKLWLG FDLRAAIAAP ILHVNSKGCV EYEPNFSQEV QRGLQDRGQN QTQRPFFLNV VQAVSQEGAC VYAVSDLRKS GEAAGY. It is sometimes possible for the material contained within the vial of "Gamma-Glutamyltransferase 5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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