Mouse anti-Human Thrombopoietin Receptor Polyclonal Antibody | anti-MPL antibody

Thrombopoietin Receptor (TPO-R, Myeloproliferative Leukemia Protein, Proto-oncogene c-Mpl, CD110, TPOR)

Cat. Num. AAA6010931

• Gene Names: MPL; MPLV; TPOR; C-MPL; CD110; THCYT2
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Thrombopoietin Receptor; Polyclonal Antibody; Thrombopoietin Receptor (TPO-R; Myeloproliferative Leukemia Protein; Proto-oncogene c-Mpl; CD110; TPOR); Anti -Thrombopoietin Receptor (TPO-R; anti-MPL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MPL.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MPSWALFMVTSCLLLAPQNLAQVSSQDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFSRCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAWISLVTALHLVLGLSAVLGLLLLRWQFPAHYRRLRHALWPSLPDLHRVLGQYLRDTAALSPPKATVSDTCEEVEPSLLEILPKSSERTPLPLCSSQAQMDYRRLQPSCLGTMPLSVCPPMAESGSCCTTHIANHSYLPLSYWQQP

• Applicable Applications for anti-MPL antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot and Flow Cytometry.
• Immunogen: Full length human MPL, aa1-635 (NP_005364).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(FACS analysis of negative control 293 cells (Black) and MPL expressing 293 cells (Green) using MPL purified mouse polyclonal antibody.)

Western Blot (WB)

(Western Blot analysis of MPL expression in transfected 293T cell line by MPL polyclonal antibody. Lane1:MPL transfected lysate (69.85KD). Lane2:Non-transfected lysate.)

Related Product Information for anti-MPL antibody

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95kD type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily.1-4 The 635aa full-length human Tpo R contains a 25aa signal sequence, a 466aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78%, 76%, 81%, 82% and 80% aa identity with mouse, rat, cow, dog, and horse, respectively.

Product Categories/Family for anti-MPL antibody

Antibodies; Abs to Thrombopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 4885491
• NCBI GeneID: 4352
• NCBI Accession #: NP_005364.1
• NCBI GenBank Nucleotide #: NM_005373.2
• UniProt Accession #: P40238; Q5JUZ0
• Molecular Weight: 71,245 Da
• NCBI Official Full Name: thrombopoietin receptor
• NCBI Official Synonym Full Names: myeloproliferative leukemia virus oncogene
• NCBI Official Symbol: MPL
• NCBI Official Synonym Symbols: MPLV; TPOR; C-MPL; CD110; THCYT2
• NCBI Protein Information: thrombopoietin receptor; TPO-R; proto-oncogene c-Mpl; myeloproliferative leukemia protein
• UniProt Protein Name: Thrombopoietin receptor
• Protein Family: Thrombopoietin receptor
• UniProt Gene Name: MPL
• UniProt Synonym Gene Names: TPOR; TPO-R
• UniProt Entry Name: TPOR_HUMAN

NCBI Description

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]

Uniprot Description

MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; transmembrane receptor activity

Biological Process: platelet activation; cell proliferation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; homeostasis of number of cells; blood coagulation; regulation of chemokine production

Disease: Myelofibrosis; Amegakaryocytic Thrombocytopenia, Congenital; Thrombocythemia 2