Goat THRA / THR Alpha Polyclonal Antibody | anti-THRA antibody

Goat Polyclonal to Human THRA / THR Alpha

Cat. Num. AAA242765

• Gene Names: THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• Reactivity: Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: THRA / THR Alpha; Polyclonal Antibody; Goat Polyclonal to Human THRA / THR Alpha; Anti-THRA / THR Alpha Antibody (Internal) IHC-plus; THRA; AR7; C-ERBA-1; C-erbaalpha; EAR-7.1/EAR-7.2; ERB-T-1; ERBA1; EAR-7; ERBA-related 7; HTR alpha 1; HTR alpha 2; THRA1; Thyroid hormone receptor alpha; T3R; Tralpha2; Triiodothyronine receptor; V-erbA-related protein 7; Thyroid hormone receptor; Human THRA; THR Alpha; anti-THRA antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
• Clonality: Polyclonal
• Specificity: Human THRA. This antibody is expected to recognize both reported isoforms (NP_955366.1; NP_003241.2). This antibody is not expected to cross-react to THRB.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 490

• Applicable Applications for anti-THRA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.5 - 1.5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KEEMIRSLQQRP from an internal region of human THRA (NP_955366.1; NP_003241.2).
• Immunogen Type: Synthetic peptide
• Immunogen: THRA / THR Alpha antibody was raised against synthetic peptide C-KEEMIRSLQQRP from an internal region of human THRA (NP_955366.1; NP_003241.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%); Chicken, Xenopus, Frog (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-THRA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-THRA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (0.5 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-THRA antibody

Thyroid hormone receptor alpha (TR alpha), a NR1 Thyroid Hormone-Like Receptor, mediates the diverse actions of thyroid hormone in development, differentiation, and metabolism. Four protein isoforms are encoded by the same TR alpha genomic locus: TR alpha1 (410 aa) (Nakai et al. 1988), TR alpha2 (490 aa) (Nakai et al. 1988), TR deltaalpha1 (~154 aa), and TR deltaalpha2 (~237 aa); however, only TR alpha1 is able to bind the ligand (Lazar et al. 1988).

Product Categories/Family for anti-THRA antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 20127452
• NCBI GeneID: 7067
• NCBI Accession #: NP_003241.2
• NCBI GenBank Nucleotide #: NM_003250.5
• UniProt Accession #: P10827; P21205; Q8N6A1; Q96H73; A8K3B5
• Molecular Weight: 50,465 Da
• NCBI Official Full Name: thyroid hormone receptor alpha isoform 2
• NCBI Official Synonym Full Names: thyroid hormone receptor, alpha
• NCBI Official Symbol: THRA
• NCBI Official Synonym Symbols: AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• NCBI Protein Information: thyroid hormone receptor alpha; EAR-7; c-erbA-alpha; ERBA-related 7; V-erbA-related protein 7; triiodothyronine receptor; nuclear receptor subfamily 1 group A member 1; thyroid normone nuclear receptor alpha variant 1; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: THRA
• UniProt Synonym Gene Names: EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
• UniProt Entry Name: THA_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: Transcription factor; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein binding; zinc ion binding; TATA-binding protein binding; chromatin DNA binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation

Disease: Hypothyroidism, Congenital, Nongoitrous, 6