Rabbit anti-Human THOC4 Polyclonal Antibody | anti-THOC4 antibody

THOC4 Conjugated Antibody

Cat. Num. AAA9445894

• Gene Names: ALYREF; ALY; BEF; REF; THOC4; ALY/REF
• Reactivity: Human
• Applications: Most Applications
• Synonyms: THOC4; Polyclonal Antibody; THOC4 Conjugated Antibody; THO complex subunit 4; Tho4; Ally of AML-1 and LEF-1; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF; anti-THOC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total THOC4 protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-THOC4 antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Synthesized peptide derived from internal of human THOC4.
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-THOC4 antibody

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.

NCBI and Uniprot Product Information

• NCBI GI #: 238776833
• NCBI GeneID: 10189
• NCBI Accession #: NP_005773.3
• NCBI GenBank Nucleotide #: NM_005782.3
• UniProt Accession #: Q86V81; O43672
• Molecular Weight: 26,888 Da
• NCBI Official Full Name: THO complex subunit 4
• NCBI Official Synonym Full Names: Aly/REF export factor
• NCBI Official Symbol: ALYREF
• NCBI Official Synonym Symbols: ALY; BEF; REF; THOC4; ALY/REF
• NCBI Protein Information: THO complex subunit 4; tho4; THO complex 4; bZIP enhancing factor; ally of AML-1 and LEF-1; bZIP-enhancing factor BEF; transcriptional coactivator Aly/REF
• UniProt Protein Name: THO complex subunit 4
• UniProt Gene Name: ALYREF
• UniProt Synonym Gene Names: ALY; BEF; THOC4; Tho4
• UniProt Entry Name: THOC4_HUMAN

NCBI Description

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

THOC4: a chaperone protein that promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains thus promoting transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Nuclear localization, translocates to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Two differentially spliced isoforms have been described.

Protein type: Spliceosome; Transcription, coactivator/corepressor; RNA-binding; Chaperone

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; nucleotide binding; single-stranded DNA binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; positive regulation of RNA elongation; regulation of DNA recombination; RNA splicing; gene expression; intronless viral mRNA export from host nucleus; mRNA 3'-end processing; termination of RNA polymerase II transcription; replication fork processing