Western Blot (WB)
(Western Blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T)
Rabbit Thioredoxin Polyclonal Antibody | anti-TXN antibody
Thioredoxin Polyclonal Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Thioredoxin; Polyclonal Antibody; Thioredoxin Polyclonal Antibody; TRX1; Trx; ADF; SASP; TXN; ATL derived factor; ATL-derived factor; DKFZp686B1993; MGC61975; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; testicular tissue protein Li 199; THIO; thioredoxin delta 3; TRDX; TRX 1; TXN delta 3; TXN protein; zgc: 92903; anti-TXN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Thioredoxin polyclonal antibody detects endogenous levels of Thioredoxin protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
85
Applicable Applications for anti-TXN antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000-1:5,000
FC/FACS: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T)
Western Blot (WB)
(Western Blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-TXN antibody
Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12kDa
NCBI Official Full Name
thioredoxin isoform 2
NCBI Official Synonym Full Names
thioredoxin
NCBI Official Symbol
TXN
NCBI Official Synonym Symbols
TRX; TRDX; TRX1
NCBI Protein Information
thioredoxin
UniProt Protein Name
Thioredoxin
Protein Family
UniProt Gene Name
TXN
UniProt Synonym Gene Names
TRDX; TRX; TRX1; Trx; ADF; SASP
UniProt Entry Name
THIO_HUMAN
NCBI Description
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on TXN
Similar Products
Product Notes
The TXN txn (Catalog #AAA3013566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thioredoxin Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Thioredoxin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:1,000-1:5,000 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TXN txn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thioredoxin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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