Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TXN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human Thioredoxin 1 Polyclonal Antibody | anti-TXN antibody
Thioredoxin 1 Rabbit pab Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity purification
Synonyms
Thioredoxin 1; Polyclonal Antibody; Thioredoxin 1 Rabbit pab Antibody; TXN; Thioredoxin 1 ; DKFZp686B1993 ; MGC61975 ; TRX ; TRX1 ; Thioredoxin ; Surfaceassociated sulphydryl protein ;; anti-TXN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
85
Applicable Applications for anti-TXN antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/100-200
Immunoprecipitation (IP): 1/50
Immunofluorescence (IF): 1/50-100
Immunohistochemistry (IHC): 1/100-200
Immunoprecipitation (IP): 1/50
Immunofluorescence (IF): 1/50-100
Immunogen
A synthetic -peptide of human Thioredoxin 1
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TXN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TXN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-TXN antibody
Rabbit polyclonal to Thioredoxin 1
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
References
1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 314. 2. BurkeGaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398404. 3. Saitoh, M. et al. (1998) EMBO J. 17, 25962606. 4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364367. 5. Callister, M.E. et al. (2006) Thorax 61, 521527.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105
NCBI Official Full Name
thioredoxin isoform 2
NCBI Official Synonym Full Names
thioredoxin
NCBI Official Symbol
TXN
NCBI Official Synonym Symbols
TRX; TRDX; TRX1
NCBI Protein Information
thioredoxin; ADF; SASP; TXN delta 3; ATL-derived factor; thioredoxin delta 3; surface-associated sulphydryl protein
UniProt Protein Name
Thioredoxin
UniProt Gene Name
TXN
UniProt Synonym Gene Names
TRDX; TRX; TRX1; Trx; ADF; SASP
UniProt Entry Name
THIO_HUMAN
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Product Notes
The TXN txn (Catalog #AAA125203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thioredoxin 1 Rabbit pab Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thioredoxin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot (WB): 1/1000-2000 Immunohistochemistry (IHC): 1/100-200 Immunoprecipitation (IP): 1/50 Immunofluorescence (IF): 1/50-100. Researchers should empirically determine the suitability of the TXN txn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thioredoxin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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