Human Ornithine Decarboxylase Antizyme 1 (OAZ1) ELISA Kit | OAZ1 elisa kit

Human Ornithine Decarboxylase Antizyme 1 (OAZ1) ELISA Kit

Cat. Num. AAA2704615

• Gene Names: OAZ1; AZ1; AZI; OAZ
• Reactivity: Human
• Synonyms: Ornithine Decarboxylase Antizyme 1 (OAZ1); Human Ornithine Decarboxylase Antizyme 1 (OAZ1) ELISA Kit; AZI; OAZ; ODC-Az; OAZ1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ornithine Decarboxylase Antizyme 1 (OAZ1).; No significant cross-reactivity or interference between Ornithine Decarboxylase Antizyme 1 (OAZ1) and analogues was observed.
• Sequence Length: 228

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Decarboxylase Antizyme 1 (OAZ1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Decarboxylase Antizyme 1 (OAZ1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OAZ1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Decarboxylase Antizyme 1 (OAZ1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Decarboxylase Antizyme 1 (OAZ1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Decarboxylase Antizyme 1 (OAZ1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Decarboxylase Antizyme 1 (OAZ1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9845505
• NCBI GeneID: 4946
• NCBI Accession #: NP_004143.1
• NCBI GenBank Nucleotide #: NM_004152.3
• UniProt Accession #: P54368; O43382; Q14989; Q92595; Q9UPL9
• Molecular Weight: 25,406 Da
• NCBI Official Full Name: ornithine decarboxylase antizyme 1 isoform 1
• NCBI Official Synonym Full Names: ornithine decarboxylase antizyme 1
• NCBI Official Symbol: OAZ1
• NCBI Official Synonym Symbols: AZ1; AZI; OAZ
• NCBI Protein Information: ornithine decarboxylase antizyme 1
• UniProt Protein Name: Ornithine decarboxylase antizyme 1
• Protein Family: Ornithine decarboxylase antizyme
• UniProt Gene Name: OAZ1
• UniProt Synonym Gene Names: OAZ; AZ1; ODC-Az

NCBI Description

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome (PubMed:17900240, PubMed:26305948, PubMed:26443277). Triggers ODC degradation by inducing the exposure of a cryptic proteasome-interacting surface of ODC (PubMed:26305948). Stabilizes AZIN2 by interfering with its ubiquitination (PubMed:17900240). Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol (PubMed:12097147).