Sheep anti-Human Thimet Oligopeptidase 1 Polyclonal Antibody | anti-THOP1 antibody
Thimet Oligopeptidase 1 (TOP1)
Gene Names
THOP1; TOP; MP78; EP24.15; MEPD_HUMAN
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by human THOP1 affinity chromatography.
Synonyms
Thimet Oligopeptidase 1; Polyclonal Antibody; Thimet Oligopeptidase 1 (TOP1); Anti -Thimet Oligopeptidase 1 (TOP1); anti-THOP1 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize human THOP1 in direct ELISAs and Western Blots. In Western Blots, this antibody shows less than 5% crossreactivity with rhNeurolysin.
Purity/Purification
Affinity Purified
Purified by human THOP1 affinity chromatography.
Purified by human THOP1 affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-THOP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 5 ng/lane under non-reducing and reducing conditions., Immunoprecipitation: This antibody has been used to immunoprecipitate rhTHOP1 from conditioned media of transfected NS0 cells., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 0.2 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rhTHOP1 (aa1-689)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-THOP1 antibody
Thimet oligopeptidase (THOP1), also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase. Widely expressed by mammalian tissues and reported to be present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme capable of hydrolyzing a number of bioactive peptides and peptides released from by the proteosome, limiting antigenic presentation by MHC class I molecules. The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat.
Product Categories/Family for anti-THOP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
78,840 Da
NCBI Official Full Name
Thimet oligopeptidase 1
NCBI Official Synonym Full Names
thimet oligopeptidase 1
NCBI Official Symbol
THOP1
NCBI Official Synonym Symbols
TOP; MP78; EP24.15; MEPD_HUMAN
NCBI Protein Information
thimet oligopeptidase; endopeptidase 24.15
UniProt Protein Name
Thimet oligopeptidase
Protein Family
UniProt Gene Name
THOP1
UniProt Entry Name
THOP1_HUMAN
Uniprot Description
THOP1: a protease that cleaves preferentially bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues. Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments.
Protein type: EC 3.4.24.15; Protease
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm
Molecular Function: protein binding; metal ion binding; metalloendopeptidase activity; peptide binding
Biological Process: peptide metabolic process; proteolysis
Research Articles on THOP1
Similar Products
Product Notes
The THOP1 thop1 (Catalog #AAA610819) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Thimet Oligopeptidase 1 (TOP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thimet Oligopeptidase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 5 ng/lane under non-reducing and reducing conditions., Immunoprecipitation: This antibody has been used to immunoprecipitate rhTHOP1 from conditioned media of transfected NS0 cells., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human THOP1. The detection limit for rhTHOP1 is approximately 0.2 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the THOP1 thop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thimet Oligopeptidase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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