Porcine Caspase Activated DNase ELISA Kit | CAD elisa kit

Porcine Caspase Activated DNase ELISA Kit

Cat. Num. AAA752388

• Gene Names: DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
• Reactivity: Porcine
• Synonyms: Caspase Activated DNase; Porcine Caspase Activated DNase ELISA Kit; CAD elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This CAD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CAD. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CAD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CAD. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAD elisa kit

Principle of the Assay: CAD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAD antibody and an CAD-HRP conjugate. The assay sample and buffer are incubated together with CAD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAD concentration since CAD from samples and CAD-HRP conjugate compete for the anti-CAD antibody binding site. Since the number of sites is limited, as more sites are occupied by CAD from the sample, fewer sites are left to bind CAD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAD concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37962942
• NCBI GeneID: 1677
• Molecular Weight: 11,482 Da
• NCBI Official Full Name: c-truncated caspase-activated DNase
• NCBI Official Synonym Full Names: DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• NCBI Official Symbol: DFFB
• NCBI Official Synonym Symbols: CAD; CPAN; DFF2; DFF40; DFF-40
• NCBI Protein Information: DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit beta
• Protein Family: CAD protein
• UniProt Gene Name: DFFB
• UniProt Synonym Gene Names: CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40
• UniProt Entry Name: DFFB_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

Uniprot Description

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; Apoptosis; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus; cytosol

Molecular Function: enzyme binding; deoxyribonuclease activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis