Western Blot (WB)
(Western Blot analysis of THG1L in HepG2 and RAMOS cell lysates in order using THG1L Rabbit pAb at dilution 1/800)
Rabbit THG1L Polyclonal Antibody | anti-THG1L antibody
THG1L Rabbit pAb
Gene Names
THG1L; ICF45; IHG-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
THG1L; Polyclonal Antibody; THG1L Rabbit pAb; tRNA-histidine guanylyltransferase 1 like; ICF45; IHG-1; hTHG1; anti-THG1L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.26mg/ml (varies by lot)
Applicable Applications for anti-THG1L antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-1:5000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
Fusion protein of human THG1L
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of THG1L in HepG2 and RAMOS cell lysates in order using THG1L Rabbit pAb at dilution 1/800)
Western Blot (WB)
(Western Blot analysis of THG1L in HepG2 and RAMOS cell lysates in order using THG1L Rabbit pAb at dilution 1/800)
Immunohistochemistry (IHC)
(Immunohistochemistry of THG1L in paraffin-embedded Human liver cancer tissue using THG1L Rabbit pAb at dilution 1/70. Predicted cell location: Cytoplasm)
Immunohistochemistry (IHC)
(Immunohistochemistry of THG1L in paraffin-embedded Human liver cancer tissue using THG1L Rabbit pAb at dilution 1/70. Predicted cell location: Cytoplasm)
Related Product Information for anti-THG1L antibody
The protein encoded by this gene is a mitochondrial protein that is induced by high levels of glucose and is associated with diabetic nephropathy. The encoded protein appears to increase mitochondrial biogenesis, which could lead to renal fibrosis. Another function of this protein is that of a guanyltransferase, adding GMP to the 5' end of tRNA(His). Several transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
298
NCBI Official Full Name
Probable tRNA(His) guanylyltransferase
NCBI Official Synonym Full Names
tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
NCBI Official Symbol
THG1L
NCBI Official Synonym Symbols
ICF45; IHG-1
NCBI Protein Information
probable tRNA(His) guanylyltransferase; probable tRNA(His) guanylyltransferase; induced by high glucose-1; interphase cytoplasmic foci protein 45
UniProt Protein Name
Probable tRNA(His) guanylyltransferase
UniProt Gene Name
THG1L
UniProt Synonym Gene Names
ICF45
UniProt Entry Name
THG1_HUMAN
Uniprot Description
ICF45: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Belongs to the tRNA(His) guanylyltransferase family.
Protein type: EC 2.7.7.79; Cell cycle regulation; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: mitochondrion; cytoplasm
Molecular Function: identical protein binding; tRNA guanylyltransferase activity; GTP binding; magnesium ion binding; ATP binding; tRNA binding
Biological Process: tRNA processing; tRNA modification; protein homotetramerization
Research Articles on THG1L
Similar Products
Product Notes
The THG1L thg1l (Catalog #AAA4759384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The THG1L Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's THG1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-1:5000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the THG1L thg1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THG1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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