Rabbit THBS1 Polyclonal Antibody | anti-THBS1 antibody

THBS1 Antibody (C-Term)

Cat. Num. AAA9231091

• Gene Names: THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
• Reactivity: Human; Predicted: Bovine
• Applications: Western Blot, ELISA
• Synonyms: THBS1; Polyclonal Antibody; THBS1 Antibody (C-Term); Thrombospondin-1; TSP; TSP1; anti-THBS1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This THBS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 820-854 amino acids from human THBS1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1170

• Applicable Applications for anti-THBS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Endoplasmic reticulum. Sarcoplasmic reticulum
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-THBS1 Antibody (C-Term) at 1:2000 dilution + MDA-MB-231 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 129 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-THBS1 antibody

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).

Product Categories/Family for anti-THBS1 antibody

Primary Antibodies; Cancer; Cardiovascular; Metabolism; Neuroscience; Stem Cells

References

Lawler J.,et al.J. Cell Biol. 103:1635-1648 (1986). Hennessy S.W.,et al.J. Cell Biol. 108:729-736 (1989). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. Zody M.C.,et al.Nature 440:671-675 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 40317626
• NCBI GeneID: 7057
• NCBI Accession #: NP_003237.2
• NCBI GenBank Nucleotide #: NM_003246.3
• UniProt Accession #: P07996; Q15667; Q59E99; A8K6H4; B4E3J7; B9EGH6
• Molecular Weight: 129383 Da
• NCBI Official Full Name: thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: THBS1
• NCBI Official Synonym Symbols: TSP; THBS; TSP1; TSP-1; THBS-1
• NCBI Protein Information: thrombospondin-1
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS1
• UniProt Synonym Gene Names: TSP; TSP1

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.

Protein type: Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; fibrinogen complex; sarcoplasmic reticulum; secretory granule

Molecular Function: fibroblast growth factor binding; fibronectin binding; heparin binding; integrin binding; laminin binding; low-density lipoprotein binding; phosphatidylserine binding; protein binding; proteoglycan binding; transforming growth factor beta binding

Biological Process: activation of MAPK activity; behavioral response to pain; cell cycle arrest; cell migration; chronic inflammatory response; engulfment of apoptotic cell; extracellular matrix organization and biogenesis; immune response; inflammatory response; negative regulation of angiogenesis; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of apoptosis; negative regulation of blood vessel endothelial cell migration; negative regulation of caspase activity; negative regulation of cell-matrix adhesion; negative regulation of dendritic cell antigen processing and presentation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of focal adhesion formation; negative regulation of interleukin-12 production; peptide cross-linking; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of macrophage activation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor-beta1 production; positive regulation of translation; positive regulation of tumor necrosis factor biosynthetic process; regulation of cGMP metabolic process; response to calcium ion; response to drug; response to glucose stimulus; response to magnesium ion; response to progesterone stimulus; sprouting angiogenesis