Goat THAP11/Ronin Polyclonal Antibody | anti-THAP11 antibody

Goat anti-THAP11/Ronin Antibody

Cat. Num. AAA422154

• Gene Names: THAP11; RONIN; CTG-B43a; CTG-B45d; HRIHFB2206
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: THAP11/Ronin; Polyclonal Antibody; Goat anti-THAP11/Ronin Antibody; THAP11; THAP domain containing 11; CTG-B43a; CTG-B45d; HRIHFB2206; RONIN; THAP domain-containing protein 11; THAP11 antibody; THAP domain containing 11 antibody; CTG-B43a antibody; CTG-B45d antibody; HRIHFB2206 antibody; RONIN antibody; THAP domain-containing protein 11 antibody; THAP11 / Ronin; anti-THAP11 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: GSDHSYSLSSGTTEE
• Sequence Length: 314

• Applicable Applications for anti-THAP11 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Western Blot: Preliminary experiments gave an approx 26kDa band in Human Umbillical Cord lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 34.5kDa according to NP_065190.2. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-GSDHSYSLSSGTTEE, from the internal region of the protein sequence according to NP_065190.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 40354197
• NCBI GeneID: 57215
• NCBI Accession #: NP_065190.2
• NCBI GenBank Nucleotide #: NM_020457.2
• Molecular Weight: 34,455 Da
• NCBI Official Full Name: THAP domain-containing protein 11
• NCBI Official Synonym Full Names: THAP domain containing 11
• NCBI Official Symbol: THAP11
• NCBI Official Synonym Symbols: RONIN; CTG-B43a; CTG-B45d; HRIHFB2206
• NCBI Protein Information: THAP domain-containing protein 11
• UniProt Protein Name: THAP domain-containing protein 11
• Protein Family: THAP domain-containing protein
• UniProt Gene Name: THAP11
• UniProt Entry Name: THA11_HUMAN

NCBI Description

The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases. [provided by RefSeq, Jul 2008]

Uniprot Description

THAP11: Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. Belongs to the THAP11 family. Interacts (via coiled coil domain) with HCFC1.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytoplasm; intercellular bridge; nucleoplasm

Molecular Function: DNA binding; protein binding; zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent