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Western Blot (WB) (Anti-TGOLN2 Antibody (C-term) at 1:1000dilution + MCF-7 whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 51 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

Rabbit TGOLN2 Polyclonal Antibody | anti-TGOLN2 antibody

TGOLN2 Antibody (C-term)

Gene Names
TGOLN2; TGN38; TGN46; TGN48; TGN51; TTGN2
Reactivity
Human
Predicted: Mouse.
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TGOLN2; Polyclonal Antibody; TGOLN2 Antibody (C-term); Trans-Golgi network integral membrane protein 2; TGN38 homolog; TGN46; TGN48; Trans-Golgi network protein TGN51; TGN51; anti-TGOLN2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Mouse.
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TGOLN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the C-terminal region of human TGOLN2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
399-427
Sequence Length
281
Applicable Applications for anti-TGOLN2 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
FC~~1:10~50
Function
May be involved in regulating membranetraffic to and from trans-Golgi network.
Cellular Location
Cell membrane; Single-pass type I membraneprotein. Golgi apparatus, trans-Golgi networkmembrane; Single- pass type I membraneprotein. Note=Primarily in trans-Golginetwork. Cycles between the trans-Golginetwork and the cell surface returning viaendosomes.
Tissue Location
Isoform TGN46 is widely expressed. IsoformTGN51 is more abundant in fetal lung andkidney. Isoform TGN48 is barely expressed inembryonic kidney and promyelocytic cells.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-TGOLN2 Antibody (C-term) at 1:1000dilution + MCF-7 whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 51 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

Western Blot (WB) (Anti-TGOLN2 Antibody (C-term) at 1:1000dilution + MCF-7 whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 51 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

Flow Cytometry (FC/FACS)

(TGOLN2 Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (TGOLN2 Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TGOLN2 antibody
TGOLN2 may be involved in regulating membrane traffic to and from trans-Golgi network.
References
Wu,C., et.al., Proteomics 7 (11), 1775-1785 (2007)
Honing,S., et.al., Mol. Cell 18 (5), 519-531 (2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
NCBI Official Full Name
trans-Golgi network integral membrane protein 2 isoform 2
NCBI Official Synonym Full Names
trans-golgi network protein 2
NCBI Official Symbol
TGOLN2
NCBI Official Synonym Symbols
TGN38; TGN46; TGN48; TGN51; TTGN2
NCBI Protein Information
trans-Golgi network integral membrane protein 2
UniProt Protein Name
Trans-Golgi network integral membrane protein 2
UniProt Gene Name
TGOLN2
UniProt Synonym Gene Names
TGN46; TGN51
UniProt Entry Name
TGON2_HUMAN

NCBI Description

This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TGOLN2: May be involved in regulating membrane traffic to and from trans-Golgi network. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; Golgi apparatus; transport vesicle; plasma membrane; integral to membrane; trans-Golgi network

Molecular Function: protein binding

Research Articles on TGOLN2

Similar Products

Product Notes

The TGOLN2 tgoln2 (Catalog #AAA9208301) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 399-427. The TGOLN2 Antibody (C-term) reacts with Human Predicted: Mouse. and may cross-react with other species as described in the data sheet. AAA Biotech's TGOLN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000 FC~~1:10~50. Researchers should empirically determine the suitability of the TGOLN2 tgoln2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGOLN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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