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Western Blot (WB) (Western blot analysis of PRDX6 Antibody (C-term) in CEM cell line lysates (35ug/lane). PRDX6 (arrow) was detected using the purified Pab.)

Rabbit anti-Human PRDX6 Polyclonal Antibody | anti-PRDX6 antibody

PRDX6, CT (Peroxiredoxin-6, Antioxidant Protein 2, 1-Cys Peroxiredoxin, 1-Cys PRX, Acidic Calcium-independent Phospholipase A2, aiPLA2, Non-selenium Glutathione Peroxidase, NSGPx, 24kD Protein, Liver 2D Page Spot 40, Red Blood Cells Page Spot 12, KIAA0106

Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
PRDX6; Polyclonal Antibody; CT (Peroxiredoxin-6; Antioxidant Protein 2; 1-Cys Peroxiredoxin; 1-Cys PRX; Acidic Calcium-independent Phospholipase A2; aiPLA2; Non-selenium Glutathione Peroxidase; NSGPx; 24kD Protein; Liver 2D Page Spot 40; Red Blood Cells Page Spot 12; KIAA0106; anti-PRDX6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRDX6.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-PRDX6 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PRDX6 (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRDX6 Antibody (C-term) in CEM cell line lysates (35ug/lane). PRDX6 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PRDX6 Antibody (C-term) in CEM cell line lysates (35ug/lane). PRDX6 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PRDX6 (arrow) using rabbit polyclonal PRDX6 Antibody (C-term) (RB20894). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).)

Western Blot (WB) (Western blot analysis of PRDX6 (arrow) using rabbit polyclonal PRDX6 Antibody (C-term) (RB20894). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PRDX6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PRDX6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PRDX6 antibody
References
1. Sorokina, E.M., et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 297(5), L871-L880 (2009). 2. Manevich, Y., et.al., Arch. Biochem. Biophys. 485(2), 139-149 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,035 Da
NCBI Official Full Name
peroxiredoxin-6
NCBI Official Synonym Full Names
peroxiredoxin 6
NCBI Official Symbol
PRDX6
NCBI Official Synonym Symbols
PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
NCBI Protein Information
peroxiredoxin-6
UniProt Protein Name
Peroxiredoxin-6
Protein Family
UniProt Gene Name
PRDX6
UniProt Synonym Gene Names
AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx
UniProt Entry Name
PRDX6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.

Protein type: Amino Acid Metabolism - phenylalanine; Oxidoreductase; Energy Metabolism - methane; EC 1.11.1.9; EC 1.11.1.15; Phospholipase

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; membrane; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol

Molecular Function: antioxidant activity; phospholipase A2 activity; protein binding; peroxiredoxin activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; phospholipid catabolic process; hydrogen peroxide catabolic process; response to oxidative stress

Research Articles on PRDX6

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Product Notes

The PRDX6 prdx6 (Catalog #AAA6492742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRDX6, CT (Peroxiredoxin-6, Antioxidant Protein 2, 1-Cys Peroxiredoxin, 1-Cys PRX, Acidic Calcium-independent Phospholipase A2, aiPLA2, Non-selenium Glutathione Peroxidase, NSGPx, 24kD Protein, Liver 2D Page Spot 40, Red Blood Cells Page Spot 12, KIAA0106 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDX6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRDX6 prdx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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