Rabbit GPR227 Polyclonal Antibody | anti-GPR227 antibody

GPR227 polyclonal antibody

Cat. Num. AAA3015002

• Gene Names: TGM3; TGE; UHS2
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR227; Polyclonal Antibody; GPR227 polyclonal antibody; Protein-glutamine gamma-glutamyltransferase E; Transglutaminase E; TG(E); TGE; TGase E; Transglutaminase-3; TGase-3; TGM3; anti-GPR227 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TGM3 polyclonal antibody detects endogenous levels of TGM3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR227 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human TGM3
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of TGM3 polyclonal antibody at 1:500 dilutionLaneA: LO2 whole cell lysate(40ug)LaneB: HEK293T whole cell lysate(40ug)LaneC: CT26 whole cell lysate(40ug)LaneD: H9C2 whole cell lysate(40ug))

Related Product Information for anti-GPR227 antibody

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.

NCBI and Uniprot Product Information

• NCBI GI #: 189458821
• NCBI GeneID: 7053
• NCBI Accession #: NP_003236.3
• NCBI GenBank Nucleotide #: NM_003245.3
• UniProt Accession #: Q08188; O95933; Q32ML9; Q32MM0; A8K5N6; B2RCR6; D3DVX1
• Molecular Weight: 76,632 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase E
• NCBI Official Synonym Full Names: transglutaminase 3
• NCBI Official Symbol: TGM3
• NCBI Official Synonym Symbols: TGE; UHS2
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase E
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase E
• UniProt Gene Name: TGM3
• UniProt Synonym Gene Names: TG(E); TGE; TGase E; TGase-3

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.