Rabbit TGFBR1 Polyclonal Antibody | anti-TGFBR1 antibody

Phospho-TGFBR1 (Ser165) Antibody

Cat. Num. AAA9614081

• Gene Names: TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: TGFBR1; Polyclonal Antibody; Phospho-TGFBR1 (Ser165) Antibody; AAT 5; AAT5; Activin A receptor type II like kinase 53kDa; Activin A receptor type II like kinase; 53kD; Activin A receptor type II like protein kinase of 53kD; activin A receptor type II-like kinase; 53kDa; activin A receptor type II-like protein kinase of 53kD; Activin receptor like kinase 5; Activin receptor-like kinase 5; ACVRLK 4; ACVRLK4; ALK 5; ALK-5; ALK5; LDS1A; LDS2A; MSSE; Serine/threonine protein kinase receptor R4; Serine/threonine-protein kinase receptor R4; SKR 4; SKR4; TbetaR I; TbetaR-I; TGF beta receptor type 1; TGF beta receptor type I; TGF beta type I receptor; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta type I receptor; TGFBR 1; TGFBR1 protein; TGFR 1; TGFR-1; TGFR1; TGFR1_HUMAN; Transforming growth factor beta receptor 1; Transforming growth factor beta receptor I (activin A receptor type II like kinase; 53kD); Transforming growth factor beta receptor I; transforming growth factor; beta receptor 1; beta receptor I (activin A receptor type II-like kinase; Transforming growth factor-beta receptor type I; anti-TGFBR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-TGFBR1 (Ser165) Antibody detects endogenous levels of TGFBR1 only when phosphorylated at TSer165.; Tissue Specificity: Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TGFBR1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated at basal levels in the absence of ligand. Activated upon phosphorylation by TGFBR2, mainly in the GS domain. Phosphorylation in the GS domain abrogates FKBP1A-binding.N-Glycosylated.Ubiquitinated; undergoes ubiquitination catalyzed by several E3 ubiquitin ligases including SMURF1, SMURF2 and NEDD4L2. Results in the proteasomal and/or lysosomal degradation of the receptor thereby negatively regulating its activity. Deubiquitinated by USP15, leading to stabilization of the protein and enhanced TGF-beta signal. Its ubiquitination and proteasome-mediated degradation is negatively regulated by SDCBP.
• Subunit Structure: Homodimer; in the endoplasmic reticulum but also at the cell membrane. Heterohexamer; TGFB1, TGFB2 and TGFB3 homodimeric ligands assemble a functional receptor composed of two TGFBR1 and TGFBR2 heterodimers to form a ligand-receptor heterohexamer. The respective affinity of TGBRB1 and TGFBR2 for the ligands may modulate the kinetics of assembly of the receptor and may explain the different biological activities of TGFB1, TGFB2 and TGFB3. Interacts with CD109; inhibits TGF-beta receptor activation in keratinocytes. Interacts with RBPMS. Interacts (unphosphorylated) with FKBP1A; prevents TGFBR1 phosphorylation by TGFBR2 and stabilizes it in the inactive conformation. Interacts with SMAD2, SMAD3 and ZFYVE9; ZFYVE9 recruits SMAD2 and SMAD3 to the TGF-beta receptor. Interacts with TRAF6 and MAP3K7; induces MAP3K7 activation by TRAF6. Interacts with PARD6A; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with SMAD7, NEDD4L, SMURF1 and SMURF2; SMAD7 recruits NEDD4L, SMURF1 and SMURF2 to the TGF-beta receptor. Interacts with USP15 and VPS39. Interacts with SDCBP (via C-terminus) Interacts with CAV1 and this interaction is impaired in the presence of SDCBP. Interacts with APPL1; interaction is TGF beta dependent; mediates trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner.
• Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Cell junction>Tight junction. Cell surface. Membrane raft.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of TGF β Receptor I (Phospho-Ser165) using Adriamycin treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-TGFBR1 antibody

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

NCBI and Uniprot Product Information

• NCBI GI #: 195963412
• NCBI GeneID: 7046
• NCBI Accession #: NP_001124388.1
• NCBI GenBank Nucleotide #: NM_001130916.1
• UniProt Accession #: P36897; Q6IR47; Q706C0; Q706C1
• Molecular Weight: 55,960 Da
• NCBI Official Full Name: TGF-beta receptor type-1 isoform 2
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor 1
• NCBI Official Symbol: TGFBR1
• NCBI Official Synonym Symbols: AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
• NCBI Protein Information: TGF-beta receptor type-1; tbetaR-I; TGF-beta receptor type I; TGF-beta type I receptor; activin receptor-like kinase 5; transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; activin A receptor type II-like kinase, 53kD
• UniProt Protein Name: TGF-beta receptor type-1
• Protein Family: TGF-beta receptor
• UniProt Gene Name: TGFBR1
• UniProt Synonym Gene Names: ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I
• UniProt Entry Name: TGFR1_HUMAN

NCBI Description

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

TGFBR1: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Found in all tissues; most abundant in placenta and least abundant in brain and heart.

Protein type: EC 2.7.11.30; Protein kinase, TKL; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Kinase, protein; Receptor, misc.; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: tight junction; membrane; cell; plasma membrane; endosome; receptor complex

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; metal ion binding; growth factor binding; receptor signaling protein activity; punt binding; SMAD binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: lens development in camera-type eye; blastocyst development; collagen fibril organization; activation of MAPKK activity; wound healing; apoptosis; regulation of protein ubiquitination; heart development; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; cell motility involved in cell locomotion; activin receptor signaling pathway; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; anterior/posterior pattern formation; regulation of transcription, DNA-dependent; extracellular structure organization and biogenesis; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; germ cell migration; angiogenesis; cell cycle arrest; kidney development; skeletal development; positive regulation of filopodium formation; pharyngeal system development; thymus development; in utero embryonic development; male gonad development; peptidyl-threonine phosphorylation; neuron fate commitment; positive regulation of cell motility; embryonic cranial skeleton morphogenesis; positive regulation of cell growth; parathyroid gland development; endothelial cell activation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mesenchymal cell differentiation; regulation of gene expression; skeletal morphogenesis; regulation of protein binding; negative regulation of endothelial cell proliferation; endothelial cell migration; epithelial to mesenchymal transition; artery morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Multiple Self-healing Squamous Epithelioma, Susceptibility To