Western Blot (WB)
(All lanes : Anti-TGFA Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HACAT whole cell lysateLane 3: RPMI 8226 whole cell lysateLane 4: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit TGFA Polyclonal Antibody | anti-TGFA antibody
TGFA Antibody (C-Term)
Gene Names
TGFA; TFGA
Reactivity
HumanPredicted: Mouse, Pig, Rat
Applications
Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
TGFA; Polyclonal Antibody; TGFA Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Protransforming growth factor alpha; Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1; anti-TGFA antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Pig, Rat
Predicted: Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This TGFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 118-150 amino acids from the human region of human TGFA.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
159
Applicable Applications for anti-TGFA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines
Cellular Location
Transforming growth factor alpha: Secreted, extracellular space
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-TGFA Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HACAT whole cell lysateLane 3: RPMI 8226 whole cell lysateLane 4: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-TGFA Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HACAT whole cell lysateLane 3: RPMI 8226 whole cell lysateLane 4: U-87 MG whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-TGFA antibody
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Product Categories/Family for anti-TGFA antibody
References
Derynck R.,et al.Cell 38:287-297(1984). Jakowlew S.B.,et al.Mol. Endocrinol. 2:1056-1063(1988). Qian J.F.,et al.Gene 132:291-296(1993). Qian J.F.,et al.Am. J. Hum. Genet. 53:168-175(1993). Machida J.,et al.Genomics 61:237-242(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17006
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha
UniProt Protein Name
Protransforming growth factor alpha
Protein Family
UniProt Gene Name
Tgfa
UniProt Synonym Gene Names
TGF-alpha; ETGF
NCBI Description
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Research Articles on TGFA
Similar Products
Product Notes
The TGFA tgfa (Catalog #AAA9231239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGFA Antibody (C-Term) reacts with Human Predicted: Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TGFA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the TGFA tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGFA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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