Rabbit TGase2 Polyclonal Antibody | anti-TGase2 antibody

TGase2 (D11) Polyclonal Antibody

Cat. Num. AAA3000943

• Gene Names: TGM2; TGC; TG(C)
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TGase2; Polyclonal Antibody; TGase2 (D11) Polyclonal Antibody; TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2; anti-TGase2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Transglutaminase 2 (D11) polyclonal antibody detects endogenous levels of Transglutaminase 2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 687

• Applicable Applications for anti-TGase2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human TGase2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TGase2 (D11) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug) Lane6:HCT116 whole cell lysate (40ug) Lane7:L02 whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TGase2 (D11) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-TGase2 antibody

-Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.

NCBI and Uniprot Product Information

• NCBI GI #: 39777597
• NCBI GeneID: 7052
• NCBI Accession #: NP_004604.2
• NCBI GenBank Nucleotide #: NP_004604.2
• UniProt Accession #: P21980
• Molecular Weight: ~ 77kDa
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase 2 isoform a
• NCBI Official Synonym Full Names: transglutaminase 2
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TGC; TG(C)
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: Transferase; EC 2.3.2.13

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of smooth muscle cell proliferation; blood vessel remodeling; protein homooligomerization; apoptotic cell clearance; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response