Western Blot (WB)
(Western blot analysis of TFIIF RAP 30 expression in SW480 (A), HepG2 (B), A549 (C) whole cell lysates.)
Rabbit anti-Human TFIIF RAP 30 Polyclonal Antibody | anti-GTF2F2 antibody
Anti-TFIIF RAP 30 Antibody
Western Blot (WB)
(Western blot analysis of TFIIF RAP 30 expression in SW480 (A), HepG2 (B), A549 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TFIIF RAP 30 expression in SW480 (A), HepG2 (B), A549 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TFIIF RAP 30 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TFIIF RAP 30 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of TFIIF RAP 30 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of TFIIF RAP 30 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family.
Protein type: EC 3.6.4.12; Helicase; Transcription initiation complex; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: holo TFIIH complex; microtubule cytoskeleton; nucleoplasm; nucleus; transcription factor TFIIF complex
Molecular Function: ATP binding; DNA binding; helicase activity; protein binding
Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcriptional preinitiation complex assembly; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
Research Articles on GTF2F2
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Product Notes
The GTF2F2 gtf2f2 (Catalog #AAA8245870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TFIIF RAP 30 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TFIIF RAP 30 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the GTF2F2 gtf2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFIIF RAP 30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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