Rabbit anti-Human TFAP2D Polyclonal Antibody | anti-TFAP2D antibody

TFAP2D, CT (TFAP2D, TFAP2BL1, Transcription factor AP-2-delta, Activating enhancer-binding protein 2-delta, Transcription factor AP-2-beta-like 1) (PE)

Cat. Num. AAA6354974

• Gene Names: TFAP2D; TFAP2BL1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TFAP2D; Polyclonal Antibody; CT (TFAP2D; TFAP2BL1; Transcription factor AP-2-delta; Activating enhancer-binding protein 2-delta; Transcription factor AP-2-beta-like 1) (PE); Transcription factor AP-2-beta-like 1; anti-TFAP2D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TFAP2D antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TFAP2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human TFAP2D.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of A549 cell stained with TFAP2D Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TFAP2D immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(TFAP2D Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TFAP2D antibody detected the TFAP2D protein (arrow).)

Related Product Information for anti-TFAP2D antibody

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC (By similarity).

Product Categories/Family for anti-TFAP2D antibody

Antibodies; Transcription Factors

References

Kim, J.M., et al. DNA Res. 13(6):275-286(2006). Wenke, A.K., et al. Biochem. Biophys. Res. Commun. 345(1):495-501(2006). Cheng, C., et al. Int. J. Biochem. Cell Biol. 34(1):78-86(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 151301211
• NCBI GeneID: 83741
• NCBI Accession #: NP_758438.2
• NCBI GenBank Nucleotide #: NM_172238.3
• UniProt Accession #: Q7Z6R9; Q8IWX0
• Molecular Weight: 49,578 Da
• NCBI Official Full Name: transcription factor AP-2-delta
• NCBI Official Synonym Full Names: transcription factor AP-2 delta
• NCBI Official Symbol: TFAP2D
• NCBI Official Synonym Symbols: TFAP2BL1
• NCBI Protein Information: transcription factor AP-2-delta
• UniProt Protein Name: Transcription factor AP-2-delta
• Protein Family: Transcription factor
• UniProt Gene Name: TFAP2D
• UniProt Synonym Gene Names: AP2-delta

Uniprot Description

AP-2 delta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Belongs to the AP-2 family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: nucleus

Biological Process: anatomical structure development; regulation of cell proliferation