Immunohistochemistry (IHC)
(MBS9602941 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit TF2L1 Polyclonal Antibody | anti-TF2L1 antibody
TF2L1 Antibody
Gene Names
TFCP2L1; LBP9; CRTR1; LBP-9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TF2L1; Polyclonal Antibody; TF2L1 Antibody; CP2 related transcriptional repressor 1; CP2-related transcriptional repressor 1; CRTR 1; CRTR-1; LBP 9; LBP9; TF2L1_HUMAN; TFCP2 L1; TFCP2L1; Transcription factor CP2 like 1; Transcription factor CP2 like protein 1; Transcription factor CP2-like protein 1; Transcription factor LBP 9; Transcription factor LBP-9; Transcription factor LBP9; anti-TF2L1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TF2L1 antibody detects endogenous levels of total TF2L1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
479
Applicable Applications for anti-TF2L1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human TF2L1
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed in placental JEG-3 cells and very low levels of expression in non-steroidogenic cells. No expression was seen in adrenal NCI-H295A cells or in adrenal tissue.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602941 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602941 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of TF2L1 using HT-29 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TF2L1 using HT-29 whole cell lysates)
Related Product Information for anti-TF2L1 antibody
Function: Transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells (ESCs) (PubMed:25215486, PubMed:26906118). With KLF2, acts as the major effector of self-renewal that mediates induction of pluripotency downstream of LIF/STAT3 and Wnt/beta-catenin signaling (By similarity). Required for normal duct development in the salivary gland and kidney (By similarity). Coordinates the development of the kidney collecting ducts intercalated (IC) and principal (PC) cells, which regulate acid-base and salt-water homeostasis, respectively (By similarity). Regulates the expression of IC genes including subunits B1 and D2 of the V-ATPase complex, OXGR1, CA12, SLC4A1, AQP6 and IC-specific transcription factor FOXI1 (By similarity). Regulates also the expression of JAG1 and subsequent notch signaling in the collecting duct (By similarity). JAG1 initiates notch signaling in PCs but inhibits notch signaling in ICs (By similarity). Acts as a transcriptional suppressor that may suppress UBP1-mediated transcriptional activation (By similarity). Modulates the placental expression of CYP11A1 (PubMed:10644752).
Subunit Structure: Forms homohexamers via its SAM-like domain (PubMed:26906118). Interacts with MTA1; which is indispensable for TFCP2l1-mediated self-renewal-promoting effect and endoderm-inhibiting action (By similarity).
Similarity: The CP2-like domain is required for direct DNA-binding (PubMed:26906118). The CP2-like domain is essential to maintain the undifferentiated state of embryonic stem cells (By similarity).The SAM-like domain is required for homohexamerization (PubMed:26906118). Belongs to the grh/CP2 family. CP2 subfamily.
Subunit Structure: Forms homohexamers via its SAM-like domain (PubMed:26906118). Interacts with MTA1; which is indispensable for TFCP2l1-mediated self-renewal-promoting effect and endoderm-inhibiting action (By similarity).
Similarity: The CP2-like domain is required for direct DNA-binding (PubMed:26906118). The CP2-like domain is essential to maintain the undifferentiated state of embryonic stem cells (By similarity).The SAM-like domain is required for homohexamerization (PubMed:26906118). Belongs to the grh/CP2 family. CP2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 55 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
transcription factor CP2-like protein 1
NCBI Official Synonym Full Names
transcription factor CP2 like 1
NCBI Official Symbol
TFCP2L1
NCBI Official Synonym Symbols
LBP9; CRTR1; LBP-9
NCBI Protein Information
transcription factor CP2-like protein 1
UniProt Protein Name
Transcription factor CP2-like protein 1
UniProt Gene Name
TFCP2L1
UniProt Synonym Gene Names
CRTR1; LBP9; CRTR-1
Uniprot Description
Transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells (ESCs) (PubMed:25215486, PubMed:26906118). With KLF2, acts as the major effector of self-renewal that mediates induction of pluripotency downstream of LIF/STAT3 and Wnt/beta-catenin signaling (). Required for normal duct development in the salivary gland and kidney (). Coordinates the development of the kidney collecting ducts intercalated (IC) and principal (PC) cells, which regulate acid-base and salt-water homeostasis, respectively (). Regulates the expression of IC genes including subunits B1 and D2 of the V-ATPase complex, OXGR1, CA12, SLC4A1, AQP6 and IC-specific transcription factor FOXI1 (). Regulates also the expression of JAG1 and subsequent notch signaling in the collecting duct (). JAG1 initiates notch signaling in PCs but inhibits notch signaling in ICs (). Acts as a transcriptional suppressor that may suppress UBP1-mediated transcriptional activation (). Modulates the placental expression of CYP11A1 (PubMed:10644752).
Research Articles on TF2L1
Similar Products
Product Notes
The TF2L1 tfcp2l1 (Catalog #AAA9602941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TF2L1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TF2L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the TF2L1 tfcp2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TF2L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
