Rabbit anti-Human TESK2 Polyclonal Antibody | anti-TESK2 antibody

TESK2, ID (C225) (Dual Specificity Testis-specific Protein Kinase 2, Testicular Protein Kinase 2) (PE)

Cat. Num. AAA6502381

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TESK2; Polyclonal Antibody; ID (C225) (Dual Specificity Testis-specific Protein Kinase 2; Testicular Protein Kinase 2) (PE); anti-TESK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TESK2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TESK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the Center (C225) region of human TESK2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-TESK2 antibody

Antibodies; Protein Kinases

References

1. Toshima, J., J. Biol. Chem. 276(33), 31449-31458 (2001). 2. Rosok, O., Genomics 61(1), 44-54 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 110349799
• NCBI GeneID: 10420
• NCBI Accession #: NP_009101
• NCBI GenBank Nucleotide #: NM_007170
• UniProt Accession #: Q96S53; Q5T422; Q5T423; Q8N520; Q9Y3Q6
• Molecular Weight: 63,639 Da
• NCBI Official Full Name: dual specificity testis-specific protein kinase 2
• NCBI Official Synonym Full Names: testis-specific kinase 2
• NCBI Official Symbol: TESK2
• NCBI Protein Information: dual specificity testis-specific protein kinase 2; testicular protein kinase 2
• UniProt Protein Name: Dual specificity testis-specific protein kinase 2
• Protein Family: Dual specificity testis-specific protein kinase
• UniProt Gene Name: TESK2
• UniProt Entry Name: TESK2_HUMAN

Uniprot Description

TESK2: a TKL kinase of the LISK family. Predominantly expressed in testis and prostate. The developmental expression pattern in the testis suggests an important role in meitoic stages and/or early stages of spermiogenesis. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; LISK family; TESK subfamily

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: focal adhesion formation; peptidyl-tyrosine phosphorylation; spermatogenesis; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation