Rabbit anti-Human TESK2 Polyclonal Antibody | anti-TESK2 antibody

TESK2, ID (C225) (Dual Specificity Testis-specific Protein Kinase 2, Testicular Protein Kinase 2) (Biotin)

Cat. Num. AAA6502373

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TESK2; Polyclonal Antibody; ID (C225) (Dual Specificity Testis-specific Protein Kinase 2; Testicular Protein Kinase 2) (Biotin); anti-TESK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TESK2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TESK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the Center (C225) region of human TESK2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TESK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). TESK2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-TESK2 antibody

Antibodies; Protein Kinases

References

1. Toshima, J., J. Biol. Chem. 276(33), 31449-31458 (2001). 2. Rosok, O., Genomics 61(1), 44-54 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 110349799
• NCBI GeneID: 10420
• NCBI Accession #: NP_009101
• NCBI GenBank Nucleotide #: NM_007170
• UniProt Accession #: Q96S53; Q5T422; Q5T423; Q8N520; Q9Y3Q6
• Molecular Weight: 63,639 Da
• NCBI Official Full Name: dual specificity testis-specific protein kinase 2
• NCBI Official Synonym Full Names: testis-specific kinase 2
• NCBI Official Symbol: TESK2
• NCBI Protein Information: dual specificity testis-specific protein kinase 2; testicular protein kinase 2
• UniProt Protein Name: Dual specificity testis-specific protein kinase 2
• Protein Family: Dual specificity testis-specific protein kinase
• UniProt Gene Name: TESK2
• UniProt Entry Name: TESK2_HUMAN

Uniprot Description

TESK2: a TKL kinase of the LISK family. Predominantly expressed in testis and prostate. The developmental expression pattern in the testis suggests an important role in meitoic stages and/or early stages of spermiogenesis. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; LISK family; TESK subfamily

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: focal adhesion formation; peptidyl-tyrosine phosphorylation; spermatogenesis; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation