Rabbit anti-Human TERT Polyclonal Antibody | anti-TERT antibody

TERT Antibody

Cat. Num. AAA9407351

• Gene Names: TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: TERT; Polyclonal Antibody; TERT Antibody; telomerase reverse transcriptase; TP2; TRT; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; anti-TERT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TERT protein.
• Form/Format: Supplied at 1.2mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 1132

• Applicable Applications for anti-TERT antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:500-1:2000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 1120-1132 amino acids of human telomerase reverse transcriptase
• Target Name: TERT
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 8%SDS-PAGELane1:Human mucinous soft sarcoma tissue lysateLane2:Human gliomas tissue lysateLane3: HT-29 cell lysateLysates: 40 ug per lanePrimary antibody: 1/600 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 40 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 31222 (TERT Antibody) at dilution 1/30, on the right is treated with the synthetic peptide.)

Related Product Information for anti-TERT antibody

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

Product Categories/Family for anti-TERT antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 109633031
• NCBI GeneID: 7015
• NCBI Accession #: NP_937983
• NCBI GenBank Nucleotide #: NM_198253.2
• Molecular Weight: 88,965 Da
• NCBI Official Full Name: telomerase reverse transcriptase isoform 1
• NCBI Official Synonym Full Names: telomerase reverse transcriptase
• NCBI Official Symbol: TERT
• NCBI Official Synonym Symbols: TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
• NCBI Protein Information: telomerase reverse transcriptase
• UniProt Protein Name: Telomerase reverse transcriptase
• Protein Family: Tertiapin
• UniProt Gene Name: TERT
• UniProt Synonym Gene Names: EST2; TCS1; TRT; TP2
• UniProt Entry Name: TERT_HUMAN

NCBI Description

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined.

Protein type: Nucleolus; Transferase; EC 2.7.7.49; DNA-binding

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolus

Molecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; nucleotidyltransferase activity; tRNA binding

Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; telomere maintenance; positive regulation of Wnt receptor signaling pathway; RNA interference, production of siRNA

Disease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1