Rabbit anti-Mouse TEF Polyclonal Antibody | anti-TEF antibody

TEF, CT (TEF, KIAA1655, Thyrotroph embryonic factor) (AP)

Cat. Num. AAA6354733

• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TEF; Polyclonal Antibody; CT (TEF; KIAA1655; Thyrotroph embryonic factor) (AP); Thyrotroph embryonic factor; anti-TEF antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TEF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the C-terminal region of human TEF.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TEF Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the TEF antibody detected the TEF protein (arrow).)

Related Product Information for anti-TEF antibody

Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-TEF antibody

Antibodies; KIAA; orf

References

Kripke, D.F., et al. Psychiatry Investig 7(1):36-42(2010). Inukai, T., et al. Blood 105(11):4437-4444(2005). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004): Newman, J.R., et al. Science 300(5628):2097-2101(2003). Hirosawa, M., et al. DNA Res. 8(1):1-9(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 223972670
• NCBI GeneID: 7008
• NCBI Accession #: NP_001138870.1
• NCBI GenBank Nucleotide #: NM_001145398.2
• UniProt Accession #: Q10587; Q15729; Q7Z3J7; Q8IU94; Q96TG4; B0QYS8; B2RC22
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: thyrotroph embryonic factor isoform 2
• NCBI Official Synonym Full Names: thyrotrophic embryonic factor
• NCBI Official Symbol: TEF
• NCBI Protein Information: thyrotroph embryonic factor
• UniProt Protein Name: Thyrotroph embryonic factor
• Protein Family: Transcription factor
• UniProt Gene Name: TEF
• UniProt Synonym Gene Names: KIAA1655
• UniProt Entry Name: TEF_HUMAN

NCBI Description

This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TEF: Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'- [TC][AG][AG]TTA[TC][AG]-3'. Belongs to the bZIP family. PAR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter