Rabbit anti-Human TECPR1 Polyclonal Antibody | anti-TECPR1 antibody

TECPR1, CT (Tectonin beta-propeller Repeat-containing Protein 1, KIAA1358) (AP)

Cat. Num. AAA6502250

• Gene Names: TECPR1; FLJ23419; FLJ90593; DKFZP434B0335
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TECPR1; Polyclonal Antibody; CT (Tectonin beta-propeller Repeat-containing Protein 1; KIAA1358) (AP); anti-TECPR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TECPR1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-TECPR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TECPR1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TECPR1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). TECPR1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain reacted with TECPR1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TECPR1 Antibody (C-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TECPR1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 32698704
• NCBI GeneID: 25851
• NCBI Accession #: NP_056210.1
• NCBI GenBank Nucleotide #: NM_015395.1
• UniProt Accession #: Q7Z6L1; Q96EB0; Q9P2I9; Q9UFR6; A8KAD1
• Molecular Weight: 129,696 Da
• NCBI Official Full Name: tectonin beta-propeller repeat-containing protein 1
• NCBI Official Synonym Full Names: tectonin beta-propeller repeat containing 1
• NCBI Official Symbol: TECPR1
• NCBI Official Synonym Symbols: FLJ23419; FLJ90593; DKFZP434B0335
• NCBI Protein Information: tectonin beta-propeller repeat-containing protein 1; OTTHUMP00000205440
• UniProt Protein Name: Tectonin beta-propeller repeat-containing protein 1
• Protein Family: Tectonin beta-propeller repeat-containing protein
• UniProt Gene Name: TECPR1
• UniProt Synonym Gene Names: KIAA1358
• UniProt Entry Name: TCPR1_HUMAN

NCBI Description

This gene encodes a tethering factor involved in autophagy. The encoded protein is found at autolysosomes, and is involved in targeting protein aggregates, damaged mitochondria, and bacterial pathogens for autophagy [provided by RefSeq, Nov 2012]

Uniprot Description

TECPR1: Tethering factor involved in autophagy. Involved in autophagosome maturation by promoting the autophagosome fusion with lysosomes: acts by associating with both the ATG5-ATG12 conjugate and phosphatidylinositol-3-phosphate (PtdIns(3)P) present at the surface of autophagosomes. Also involved in selective autophagy against bacterial pathogens, by being required for phagophore/preautophagosomal structure biogenesis and maturation. Interacts with ATG5; the interaction is direct. Interacts with WIPI2. Interacts with the ATG5-ATG12 conjugate, the interaction is however mutually exclusive with ATG16, since it does not interact with ATG12-ATG5-ATG16 complex. Belongs to the TECPR1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: lysosomal membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: protein binding; phosphatidylinositol 3-phosphate binding

Biological Process: autophagic vacuole fusion; autophagy