Rabbit anti-Human TDT Polyclonal Antibody | anti-TDT antibody

TDT, NT (DNTT, TDT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase) (FITC)

Cat. Num. AAA6354670

• Gene Names: DNTT; TDT
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TDT; Polyclonal Antibody; NT (DNTT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase) (FITC); DNTT; Terminal deoxynucleotidyltransferase; anti-TDT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TDT antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human TDT.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TdT Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the TdT antibody detected the TdT protein (arrow).)

Related Product Information for anti-TDT antibody

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Product Categories/Family for anti-TDT antibody

Antibodies; Enzymes

References

Kubota, T., et al. Genes Cells 12(8):941-959(2007). O'Malley, D.P., et al. Haematologica 91(8):1139-1140(2006). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Thai, T.H., et al. J. Immunol. 173(6):4009-4019(2004). Liu, L., et al. Am. J. Clin. Pathol. 121(6):810-815(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 63054852
• NCBI GeneID: 1791
• NCBI Accession #: NP_001017520.1; NP_004079.3
• NCBI GenBank Nucleotide #: NM_001017520.1
• UniProt Accession #: P04053; Q53FH1; Q5W103; Q96E50
• Molecular Weight: 58,536 Da
• NCBI Official Full Name: DNA nucleotidylexotransferase isoform 2
• NCBI Official Synonym Full Names: DNA nucleotidylexotransferase
• NCBI Official Symbol: DNTT
• NCBI Official Synonym Symbols: TDT
• NCBI Protein Information: DNA nucleotidylexotransferase; terminal transferase; terminal addition enzyme; terminal deoxynucleotidyltransferase; deoxynucleotidyltransferase, terminal; terminal deoxyribonucleotidyltransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase
• UniProt Protein Name: DNA nucleotidylexotransferase
• Protein Family: Tonoplast dicarboxylate transporter
• UniProt Gene Name: DNTT
• UniProt Synonym Gene Names: TDT; Terminal transferase
• UniProt Entry Name: TDT_HUMAN

NCBI Description

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.31

Chromosomal Location of Human Ortholog: 10q23-q24

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: DNA binding; metal ion binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity

Biological Process: DNA modification