Rabbit TCP-1theta Polyclonal Antibody | anti-TCP-1theta antibody

TCP-1theta Polyclonal Antibody

Cat. Num. AAA3006262

• Gene Names: CCT8; Cctq; PRED71; D21S246; C21orf112
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TCP-1theta; Polyclonal Antibody; TCP-1theta Polyclonal Antibody; T-complex protein 1 subunit theta; TCP1theta; TCP-1 theta; TCP1 theta; TCP1-theta; TCP-1-theta; CCT-theta; CCTtheta; Renal carcinoma antigen NY-REN-15; CCT8; C21orf112; CCTQ; KIAA0002; anti-TCP-1theta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TCP-1theta polyclonal antibody detects endogenous levels of TCP-1theta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 548

• Applicable Applications for anti-TCP-1theta antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 404-449 of Human TCP-1theta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TCP-1theta polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysateLane2:NIH-3T3 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-TCP-1theta antibody

The protein TCP-1 (t complex polypeptide 1) is a subunit of the heterooligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 alpha, beta, gamma, delta, epsilon, zeta, eta and theta, each encoded by a different gene. Two zeta subunits have been described: TCP-1 zeta (also designated TCP-1 zeta1) and TCP-1 zeta2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 48762932
• NCBI GeneID: 10694
• NCBI Accession #: NP_006576.2
• NCBI GenBank Nucleotide #: NP_006576.2
• UniProt Accession #: P50990
• Molecular Weight: ~ 59kDa
• NCBI Official Full Name: T-complex protein 1 subunit theta isoform 1
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 8
• NCBI Official Symbol: CCT8
• NCBI Official Synonym Symbols: Cctq; PRED71; D21S246; C21orf112
• NCBI Protein Information: T-complex protein 1 subunit theta
• UniProt Protein Name: T-complex protein 1 subunit theta
• UniProt Gene Name: CCT8
• UniProt Synonym Gene Names: C21orf112; CCTQ; KIAA0002; TCP-1-theta
• UniProt Entry Name: TCPQ_HUMAN

NCBI Description

This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013]

Uniprot Description

CCT-theta: a chaperone of the TCP-1 chaperonin family. A part of a hetero-oligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

Protein type: Chaperone; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; intermediate filament cytoskeleton; cytoplasm; cytosol

Molecular Function: protein binding; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis