Rabbit TCP-1epsilon Polyclonal Antibody | anti-TCP-1epsilon antibody

TCP-1epsilon Polyclonal Antibody

Cat. Num. AAA3006258

• Gene Names: CCT5; CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TCP-1epsilon; Polyclonal Antibody; TCP-1epsilon Polyclonal Antibody; T-complex protein 1 subunit epsilon; TCP-1-epsilon; TCP1epsilon; TCP 1epsilon; TCP1-epsilon; CCT-epsilon; CCTepsilon; CCT5; CCTE; KIAA0098; anti-TCP-1epsilon antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TCP-1epsilon polyclonal antibody detects endogenous levels of TCP-1epsilon protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 541

• Applicable Applications for anti-TCP-1epsilon antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 295-340 of Human TCP-1epsilon.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TCP-1epsilon polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-TCP-1epsilon antibody

The protein TCP-1 (t complex polypeptide 1) is a subunit of the heterooligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 alpha, beta, gamma, delta, epsilon, zeta, eta and theta, each encoded by a different gene. Two zeta subunits have been described: TCP-1 zeta (also designated TCP-1 zeta1) and TCP-1 zeta2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 24307939
• NCBI GeneID: 22948
• NCBI Accession #: NP_036205.1
• NCBI GenBank Nucleotide #: NP_036205.1
• UniProt Accession #: P48643
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: T-complex protein 1 subunit epsilon isoform a
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 5
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098; TCP-1-epsilon
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive