Goat anti-Mouse Tcl1 Polyclonal Antibody | anti-TCL1A antibody

Tcl1 (Tcl1, T-cell lymphoma breakpoint 1)

Cat. Num. AAA622930

• Gene Names: TCL1A; TCL1
• Reactivity: Mouse
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Tcl1; Polyclonal Antibody; Tcl1 (Tcl1; T-cell lymphoma breakpoint 1); Anti -Tcl1 (Tcl1; anti-TCL1A antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TCL1. Sequence homology: mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:16,000
• Immunogen: Synthetic peptide corresponding to QLYPKDRYRSCD, from the internal region of TCL1 (NP_033363.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TCL1A antibody

Most lymphomas arise by transformation of germinal center (GC) B cells. TCL1, a proto-oncogene first recognized for its role in T-cell transformation, also induces GC B-cell malignancies when dysregulated in pEmu-B29-TCL1 transgenic (TCL1-tg) mice. Clonal B-cell lymphomas develop from polyclonal populations with latencies of 4 months or more, suggesting that secondary genetic events are required for full transformation. The goals of this study were to determine the GC-related effects of TCL1 dysregulation that contribute to tumor initiation and to identify companion genetic alterations in tumors that function in disease progression. We report that compared with wild-type (WT) cells, B cells from TCL1-tg mice activated in a manner resembling a T-dependent GC reaction show enhanced resistance to FAS-mediated apoptosis with CD40 stimulation, independent of a B-cell antigen receptor (BCR) rescue signal. Mitogenic stimulation of TCL1-tg B cells also resulted in increased expression of Aicda. These GC-related enhancements in survival and Aicda expression could underlie B-cell transformation. Supporting this notion, no B-cell lymphomas developed for 20 months when TCL1-tg mice were crossed onto an Oct coactivator from B cell (OCA-B)-deficient background to yield mice incapable of forming GCs. Spectral karyotype analyses showed that GC lymphomas from TCL1-tg mice exhibit recurrent chromosome translocations and trisomy 15, with corresponding MYC overexpression. We conclude that pEmu-B29-TCL1 transgenic B cells primed for transformation must experience the GC environment and, for at least some, develop genome instability to become fully malignant.

Product Categories/Family for anti-TCL1A antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 148922879
• NCBI GeneID: 8115
• NCBI Accession #: NP_001092195.1
• NCBI GenBank Nucleotide #: NM_001098725.1
• UniProt Accession #: P56279; Q6IBK7
• Molecular Weight: 13,460 Da
• NCBI Official Full Name: T-cell leukemia/lymphoma protein 1A
• NCBI Official Synonym Full Names: T-cell leukemia/lymphoma 1A
• NCBI Official Symbol: TCL1A
• NCBI Official Synonym Symbols: TCL1
• NCBI Protein Information: T-cell leukemia/lymphoma protein 1A; oncogene TCL1; oncogene TCL-1; protein p14 TCL1; T-cell lymphoma-1
• UniProt Protein Name: T-cell leukemia/lymphoma protein 1A
• Protein Family: T-cell leukemia/lymphoma protein
• UniProt Gene Name: TCL1A
• UniProt Synonym Gene Names: TCL1; Oncogene TCL1
• UniProt Entry Name: TCL1A_HUMAN

NCBI Description

Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al., 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al., 2000 [PubMed 10983986]).[supplied by OMIM, Jul 2010]

Uniprot Description

TCL1A: Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. Homodimer. Interacts with AKT1, AKT2 and AKT3 (via PH domain). Interacts with PNPT1; the interaction has no effect on PNPT1 exonuclease activity. Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. Belongs to the TCL1 family.

Protein type: Protein kinase, regulatory subunit; Oncoprotein

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: pronucleus; endoplasmic reticulum; cell cortex

Molecular Function: protein binding

Biological Process: multicellular organismal development; stem cell maintenance