Rabbit anti-Rat TCF7L2 Polyclonal Antibody | anti-TCF7L2 antibody

TCF7L2 Antibody: ATTO 390

Cat. Num. AAA8000428

• Gene Names: TCF7L2; TCF4; TCF-4
• Reactivity: Rat
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: TCF7L2; Polyclonal Antibody; TCF7L2 Antibody: ATTO 390; Rabbit Anti-Human TCF7L2 Polyclonal; Transcription factor 7-like 2 antibody; HMG box transcription factor 4 antibody; hTCF 4 antibody; hTCF-4 antibody; T cell factor 4 antibody; T cell specific HMG box antibody; T cell specific transcription factor 4 antibody; T-cell factor 4 antibody; T-cell-specific transcription factor 4 antibody; TCF 4 antibody; TCF-4 antibody; TCF4 antibody; TCF7L2 antibody; TCF7L2 protein antibody; TF7L2_HUMAN antibody; Transcription factor 7 like 2 antibody; Transcription factor 7 like 2 T cell specific HMG box antibody; anti-TCF7L2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Detects 68 kDa, band at ~55 kDa is another isoform, band at ~75 kDa is due to post translational modification.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 602

• Applicable Applications for anti-TCF7L2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: TCF7L2
• Immunogen: Synthetic peptide of Human TCF7L2 (400-500 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Immunogen Species: Human
• Cellular Localization: Nucleus; Pml Body
• Conjugate: ATTO 390
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)

Product Categories/Family for anti-TCF7L2 antibody

Antibodies; Cell Signaling; Epigenetics and Nuclear Signaling; Transcription; Domain Families; Hmg box; Cardiovascular System; Atherosclerosis; Diabetes Associated; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 226371764
• NCBI GeneID: 6934
• NCBI Accession #: NP_001139746.1
• NCBI GenBank Nucleotide #: NM_001146274.1
• UniProt Accession #: Q9NQB0; B4DRJ8; B9X074; C6ZRJ8; C6ZRK0; E2GH14; E2GH19; E2GH20; E2GH24; E2GH25; E9PFH9; F8W742
• Molecular Weight: 36,884 Da
• NCBI Official Full Name: transcription factor 7-like 2 isoform 1
• NCBI Official Synonym Full Names: transcription factor 7 like 2
• NCBI Official Symbol: TCF7L2
• NCBI Official Synonym Symbols: TCF4; TCF-4
• NCBI Protein Information: transcription factor 7-like 2
• UniProt Protein Name: Transcription factor 7-like 2
• Protein Family: Transcription factor
• UniProt Gene Name: TCF7L2
• UniProt Synonym Gene Names: TCF4; T-cell factor 4; TCF-4; hTCF-4

NCBI Description

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.