Western Blot (WB)
(Western blot analysis of NKIRAS2 expression in A549 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse NKIRAS2 Polyclonal Antibody | anti-NKIRAS2 antibody
NKIRAS2 Antibody
Gene Names
NKIRAS2; KBRAS2; kappaB-Ras2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NKIRAS2; Polyclonal Antibody; NKIRAS2 Antibody; DKFZP434N1526; I kappa B interacting Ras like protein 2; I-kappa-B-interacting Ras-like protein 2; Kappa B Ras protein 2; Kappa B-Ras protein 2; KappaB Ras2; KappaB-Ras2; KBRAS 2; KBRAS2; KBRS2_HUMAN; MGC74742; NF kappa B inhibitor interacting Ras like protein 2; NF-kappa-B inhibitor-interacting Ras-like protein 2; NFKB inhibitor interacting Ras like 2; NFKB inhibitor interacting Ras like protein 2; NKIRAS 2; anti-NKIRAS2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NKIRAS2 antibody detects endogenous levels of total NKIRAS2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
191
Applicable Applications for anti-NKIRAS2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm.
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Zebrafish (85%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of NKIRAS2 expression in A549 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of NKIRAS2 expression in A549 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-NKIRAS2 antibody
Description: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP-and GDP-bound forms block phosphorylation of NFKBIB.
Function: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP-and GDP-bound forms block phosphorylation of NFKBIB (By similarity).
Subunit Structure: Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo.
Similarity: In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
Function: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP-and GDP-bound forms block phosphorylation of NFKBIB (By similarity).
Subunit Structure: Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo.
Similarity: In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform a
NCBI Official Synonym Full Names
NFKB inhibitor interacting Ras like 2
NCBI Official Symbol
NKIRAS2
NCBI Official Synonym Symbols
KBRAS2; kappaB-Ras2
NCBI Protein Information
NF-kappa-B inhibitor-interacting Ras-like protein 2
UniProt Protein Name
NF-kappa-B inhibitor-interacting Ras-like protein 2
Protein Family
UniProt Gene Name
NKIRAS2
UniProt Synonym Gene Names
KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
Uniprot Description
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB ().
Research Articles on NKIRAS2
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Product Notes
The NKIRAS2 nkiras2 (Catalog #AAA9604094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKIRAS2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NKIRAS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NKIRAS2 nkiras2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKIRAS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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